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MABE1056

Sigma-Aldrich

Anti-ZBTB7A Antibody, clone 13E9

clone 13E9, from hamster(Armenian)

Sinónimos:

Zinc finger and BTB domain-containing protein 7A, Factor binding IST protein 1, FBI-1, Factor that binds to inducer of short transcripts protein 1, HIV-1 1st-binding protein 1, Leukemia/lymphoma-related factor, POZ and Krueppel erythroid myeloid ontogeni

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

hamster (Armenian)

Nivel de calidad

forma del anticuerpo

purified antibody

tipo de anticuerpo

primary antibodies

clon

13E9, monoclonal

reactividad de especies

human, mouse

técnicas

immunohistochemistry: suitable (paraffin)
western blot: suitable

isotipo

IgG

Nº de acceso NCBI

Nº de acceso UniProt

Condiciones de envío

wet ice

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... ZBTB7A(51341)

Descripción general

Zinc finger and BTB domain-containing protein 7A (UniProt O95365; also known as Factor binding IST protein 1, Factor that binds to inducer of short transcripts protein 1, FBI-1, HIV-1 1st-binding protein 1, Leukemia/lymphoma-related factor, POK erythroid myeloid ontogenic factor, Pokemon, TIP21, POZ and Krueppel erythroid myeloid ontogenic factor, TTF-I-interacting peptide 21, Zinc finger and BTB domain-containing protein 7A, Zinc finger protein 857A) is encoded by the ZBTB7A (also known as FBI1, LRF, ZBTB7, ZNF857A) gene (Gene ID 51341) in human. Pokemon/ZBTB7A belongs to the family of POK (POZ/BTB and Krüppel) transcription factors that play important roles in cellular differentiation and oncogenesis. Pokemon/ZBTB7A overexpression is reported in cancers of various origins, including non–small-cell lung cancer (NSCLC), breast cancer, ovarian cancer, as well as in non-Hodgkin lymphoma, where it is found to directly repress the expression of the tumor suppressor ARF. On the other hand, loss of pokemon/ZBTB7A expression is also found to promote the progression of prostate tumorigenesis among a subset of human advanced prostate cancers by bypassing Pten loss–induced cellular senescence (PICS) as a result of Sox9-dependent oncogenic pathways activation.

Especificidad

Clone 13E9 detected pokemon/ZBTB7A expression in wild-type, but not Zbtb7-/-, mouse embryonic fibroblasts by Western blotting and showed strong and specific nuclear staining only in Zbtb7+/+ fetal liver (FL) cells, but not in Zbtb7-/- FL cells (Maeda, T., et al. (2005). Nature. 433(7023):278-285).

Inmunógeno

Linear peptide corresponding to the N-terminal sequence of human ZBTB7A.

Aplicación

Anti-ZBTB7A Antibody, clone 13E9 is an antibody against ZBTB7A for use in Western Blotting, Immunohistochemistry (Paraffin).
Western Blotting Analysis: 2.0 µg/mL from a representative lot detected ZBTB7A in 10 µg of HepG2 and K562 cell lysates.
Western Blotting Analysis: A representative lot detected much lower pokemon/ZBTB7A exprerssion levels in 6 human prostate cell lines when compared with the non-tumourigenic RWPE-1 prostate epithelial cell line (Wang, G., et al. (2013). Nat. Genet. 45(7):739-746).
Western Blotting Analysis: A representative lot detected a downregulated pokemon/ZBTB7A exprerssion in human DU145 prostate cancer cells transfected with miR-106b or miR-93, but not miR-25 (Wang, G., et al. (2013). Nat. Genet. 45(7):739-746).
Western Blotting Analysis: A representative lot detected pokemon/ZBTB7A expression in prostates from wild-type mice, but not transgenic mice with prostate-specific Zbtb7a gene-knockout (Wang, G., et al. (2013). Nat. Genet. 45(7):739-746).
Western Blotting Analysis: A representative lot detected pokemon/ZBTB7A expression in wild-type mouse thymocytes and mEFs, but not Zbtb7-/- mEFs. Clone13E9 also detected the overexpressed pokemon levels in thymic lymphomas developed among lckEµ-Pokemon transgenic mice (Maeda, T., et al. (2005). Nature. 433(7023):278-285).
Immunohistochemistry Analysis: A representative lot detected a positive correlation between the pokemon/ZBTB7A immunoreactivity and that of PTEN in formalin-fixed, paraffin-embedded human prostate cancer tissues (Wang, G., et al. (2013). Nat. Genet. 45(7):739-746).
Immunohistochemistry Analysis: A representative lot detected nuclear pokemon/ZBTB7A immunoreactivity in human (thymus and tonsil) and mouse (thymus and spleen) lymphoid organs using paraffin-embedded tissue sections (Maeda, T., et al. (2005). Nature. 433(7023):278-285).
Immunohistochemistry Analysis: A representative lot detected specific nuclear pokemon/ZBTB7A immunoreactivity only in Zbtb7+/+ fetal liver (FL) cells, but not in Zbtb7-/- FL cells (Maeda, T., et al. (2005). Nature. 433(7023):278-285).
Immunohistochemistry Analysis: A representative lot detected pokemon/ZBTB7A immunoreactivity among subsets of human T-cell lymphomas, diffuse large B-cell lymphoma (DLBCL), and follicular lymphoma (FL) samples, while little pokemon immunoreactivity was seen in normal human thymic CD3+ cells (Maeda, T., et al. (2005). Nature. 433(7023):278-285).

Calidad

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected ZBTB7A in 10 µg of HeLa cell lysate.

Descripción de destino

~70 kDa observed. Target band appears larger than the calculated molecular weight of 61.4 kDa

Forma física

Format: Purified

Otras notas

Concentration: Please refer to lot specific datasheet.

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Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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