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Merck
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MABC128

Sigma-Aldrich

Anti-FIP200 Antibody, clone 14E11.2

clone 14E11.2, from mouse

Sinónimos:

RB1-inducible coiled-coil protein, 1FAK family kinase-interacting protein of 200 kDa, FIP200

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

100

forma del anticuerpo

purified antibody

tipo de anticuerpo

primary antibodies

clon

14E11.2, monoclonal

reactividad de especies

human, mouse

técnicas

immunocytochemistry: suitable
western blot: suitable

isotipo

IgG2bκ

Nº de acceso NCBI

NP_001077086

Nº de acceso UniProt

Q8TDY2

Condiciones de envío

wet ice

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... RB1CC1(9821)

Descripción general

Focal adhesion kinase family interacting protein of 200 kDa (FIP200) is a novel protein inhibitor for focal adhesion kinase (FAK). FIP200 inhibits kinase activity and associated cellular functions, such as cell adhesion, spreading, and motility in fibroblasts by binding directly to FAK.

The previously assigned protein identifier Q86YR4 has been merged into Q8TDY2. Full details can be found on the UniProt database.

Especificidad

This antibody binds to the interaction domain of FIP200, which is needed for the formation of the ULK-FIP200 complex under starved, autophagy conditions.

Inmunógeno

Epitope: Interaction domain
GST-tagged recombinant protein corresponding to the interaction domain of human FIP200.

Aplicación

Anti-FIP200 Antibody, clone 14E11.2 is an antibody against FIP200 for use in Western Blotting, ICC.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Western Blot Analysis: 1 µg/mL from a representative lot detected FIP200 in serum starved and non-serum starved HeLa cell lysate.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected FIP200 in NIH/3T3, A431, and HeLa cells.

Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected FIP200 in untreated and chloroquinone-treated HUVEC cells.

Calidad

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected FIP200 in 10 µg of HeLa cell lysate.

Descripción de destino

~200 kDa observed. An uncharacterized band may be observed at ~50 kDa in some cell lysates.

Forma física

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Nota de análisis

Control
HeLa cell lysate

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Nature communications, 12(1), 3292-3292 (2021-06-04)
Autophagy regulates primary cilia formation, but the underlying mechanism is not fully understood. In this study, we identify NIMA-related kinase 9 (NEK9) as a GABARAPs-interacting protein and find that NEK9 and its LC3-interacting region (LIR) are required for primary cilia
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Nature communications, 7, 10884-10884 (2016-03-11)
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Autophagy is a highly regulated process that is involved in cell growth, development, and death. In autophagy cells destroy their own cytoplasmic components in a very systematic manner and recycle them.

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