MABC1176M

Anti-Bax Antibody, clone 6A7

clone 6A7, from mouse

• Sinónimos: Apoptosis regulator BAX, Bcl-2-like protein 4, Bcl2-L-4
• Código UNSPSC: 12352203
• eCl@ss: 32160702

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: 6A7, monoclonal
• reactividad de especies: human
• reactividad de especies (predicha por homología): rat (based on 100% sequence homology), mouse (based on 100% sequence homology)
• técnicas: immunofluorescence: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotipo: IgG1κ
• Nº de acceso NCBI: NP_001278357
• Nº de acceso UniProt: Q07812
• Condiciones de envío: ambient
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... BAX(581)

Descripción

Descripción general

Apoptosis regulator Bax (UniProt: Q07812; also known as Bcl-2-like protein 4, Bcl2-L-4) is encoded by the BAX (also known as BCL2L4) gene (Gene ID: 581) in human. Bax is a pro-apoptotic member of the Bcl-2 family of proteins. It displays extensive homology with Bcl-2 and is variably expressed in different cells, usually at very low concentrations in normal cells. Upon induction of apoptosis Bax undergoes a conformational change and translocates from cytosol to mitochondrial membrane. Bax forms homodimers as well as heterodimers with Bcl-2 through common binding motif and the ratio of Bax to Bcl-2 determines the survival or death of cells during apoptosis. BAX is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. Clone 6A7 reacts with multiple isoforms of Bax.

Especificidad

Clone 6A7 recognizes BAX in human cells. It targets an epitope with in 13 amino acids from the N-terminal region.

Inmunógeno

KLH-cojugated linear peptide coprresponding to 13 amino acids from the N-terminal region of human BAX.

Aplicación

Anti-Bax, clone 6A7, Cat. No. MABC1176, is a mouse monoclonal antibody that detects Bax and has been tested for use in Immunofluorescence, Immunoprecipitation, and Western Blotting.

Research Category
Apoptosis & Cancer

Western Blotting Analysis: A representative lot detected Bax in Western Blotting applications (Xin, M., et. al. (2014). Nat Commun. 5:4935).

Immunofluorescence Analysis: A representative lot detected Bax in Immunofluorescence applications (Xin, M., et. al. (2014). Nat Commun. 5:4935).

Immunoprecipitation Analysis:A representative lot detected Bax in Immunoprecipitation applications (Xin, M., et. al. (2014). Nat Commun. 5:4935; Hsu, Y.T., et. al. (1998). J Biol Chem. 273(17):10777-83).

Calidad

Evaluated by Immunoprecipitation in THP-1 cell lysates.

Immunoprecipitation Analysis: A 1:250 dilution of this antibody detected Bax in THP-1 cell lysates.

Descripción de destino

~15 kDa observed; 21.18 kDa calculated. Uncharacterized bands may be observed in some lysate(s). Multiple isoforms for this target (8), ranging in MWs between 4-24 kDa, are reported. Most of the predicted MWs reside between 12-19 kDa Based on the sequence it is expected to react with isoforms 1,2,4,5, and 8.

Forma física

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable