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Key Documents

MAB4601

Sigma-Aldrich

Anti-Bax Antibody, NT, aa3-16 hBax protein, clone 2D2

clone 2D2, Chemicon®, from mouse

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

forma del anticuerpo

purified immunoglobulin

tipo de anticuerpo

primary antibodies

clon

2D2, monoclonal

reactividad de especies

human

fabricante / nombre comercial

Chemicon®

técnicas

immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

isotipo

IgG1

Nº de acceso UniProt

Condiciones de envío

wet ice

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... BAX(581)

Descripción general

The previously assigned protein identifier Q07814 has been merged into Q07812. Full details can be found on the UniProt database.

Especificidad

Recognizes a 21 kD protein. Does not cross-react with Bcl-2a or Bcl-X. In contrast to Bcl-2a and Bcl-X, overexpression of Bax protein accelerates apoptosis.

Inmunógeno

Epitope: N-terminus, aa3-16 hBax protein
Synthetic peptide corresponding to aa 3-16 (GSGEQPRGGGPTSS) of human bax. This amino acid sequence is not shared by mouse or rat Bax.

Aplicación

Anti-Bax Antibody, N-terminus, aa3-16 hBax protein, clone 2D2 is an antibody against Bax for use in IF, WB, IH(P).
Research Category
Apoptosis & Cancer
Research Sub Category
BCL2 & Inhibition
Western blot: 1 μg/ml for 2 hours at RT (1:200 dilution)

Immunofluorescence

Immunohistochemistry (frozen and paraffin): 2-4 μg/ml for 30 minutes at RT

(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min. followed by cooling for 20 min. at RT)

Optimal working dilutions must be determined by end user.

Ligadura / enlace

Replaces: 04-434

Forma física

Format: Purified
Liquid in 10 mM PBS, pH 7.4, with 0.2% BSA and 15 mM sodium azide.

Almacenamiento y estabilidad

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Opa1-mediated cristae opening is Bax/Bak and BH3 dependent, required for apoptosis, and independent of Bak oligomerization.
Yamaguchi, R; Lartigue, L; Perkins, G; Scott, RT; Dixit, A; Kushnareva, Y; Kuwana et al.
Molecular Cell null
Sabrina L Spencer et al.
Nature, 459(7245), 428-432 (2009-04-14)
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