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MAB4388

Sigma-Aldrich

Mouse Anti-Stella Antibody

clone 3H5.2, Chemicon®, from mouse

Sinónimos:

Anti-Anti-Pgc7, Anti-Anti-STELLA

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
clon:
3H5.2, monoclonal
application:
ELISA
ICC
reactividad de especies:
mouse, human
técnicas:
ELISA: suitable
immunocytochemistry: suitable
citations:
10

origen biológico

mouse

Nivel de calidad

forma del anticuerpo

purified immunoglobulin

tipo de anticuerpo

primary antibodies

clon

3H5.2, monoclonal

reactividad de especies

mouse, human

fabricante / nombre comercial

Chemicon®

técnicas

ELISA: suitable
immunocytochemistry: suitable

entrada

sample type: human embryonic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type: mouse embryonic stem cell(s)

isotipo

IgG1

Nº de acceso NCBI

Nº de acceso UniProt

Condiciones de envío

wet ice

modificación del objetivo postraduccional

unmodified

Información sobre el gen

mouse ... Dppa3(73708)

Descripción general

Stella is specifically expressed by oocytes, pluripotent cells and primordial germ cells at the time of their emergence in gastrulating embryos, and is an important maternal factor in the cleavage stages of mouse embryos (Bortvin, 2004). The requirement for stella in germ cell specification remains controversial, however, most embryos from stella deficient mice failed to develop normally and rarely reach the blastocyst stage (Bortvin, 2004; Payer, 2003). The stella gene encodes a protein with an SAP-like domain and splicing factor motif-like structure, suggesting possible roles in chromosomal organization and RNA processing (Payer, 2003).

Especificidad

Reacts with stella from human and mouse.

Aplicación

Mouse Anti-Stella Antibody detects level of Stella & has been published & validated for use in ELISA & IC.
Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation

Forma física

Format: Purified
Purified Immunoglobulin presented as a liquid in PBS with 0.1% sodium azide.

Almacenamiento y estabilidad

Maintain refrigerated at 2-8C in undiluted aliquots for up to 12 months.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Human primordial germ cell commitment in vitro associates with a unique PRDM14 expression profile.
Sugawa, F; Arauzo-Bravo, MJ; Yoon, J; Kim, KP; Aramaki, S; Wu, G; Stehling, M; Psathaki et al.
The Embo Journal null
Alex Bortvin et al.
BMC developmental biology, 4, 2-2 (2004-03-17)
In mice, germ cells are specified through signalling between layers of cells comprising the primitive embryo. The function of Dppa3 (also known as Pgc7 or stella), a gene expressed in primordial germ cells at the time of their emergence in
Generation of stella-GFP transgenic mice: a novel tool to study germ cell development
Payer, Bernhard, et al
Genesis, 44, 75-83 (2006)
Bernhard Payer et al.
Current biology : CB, 13(23), 2110-2117 (2003-12-05)
stella is a novel gene specifically expressed in primordial germ cells, oocytes, preimplantation embryos, and pluripotent cells. It encodes a protein with a SAP-like domain and a splicing factor motif-like structure, suggesting possible roles in chromosomal organization or RNA processing.
Katja Linher et al.
PloS one, 4(12), e8263-e8263 (2009-12-17)
We have previously demonstrated that stem cells isolated from fetal porcine skin have the potential to form oocyte-like cells (OLCs) in vitro. However, primordial germ cells (PGCs), which must also be specified during the stem cell differentiation to give rise

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