MAB342-AF555

Anti-Galactocerebroside Antibody, clone mGalC Antibody, Alexa Fluor^™ 555 conjugate

clone mGalC, from mouse, ALEXA FLUOR^™ 555

• Sinónimos: Galactocerebrocide, Galactosylceramide, GalC, GalCer
• Código UNSPSC: 12352203
• eCl@ss: 32160702

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• conjugado: ALEXA FLUOR^™ 555
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: mGalC, monoclonal
• reactividad de especies: rabbit, rat, mouse, bovine, human
• técnicas: immunocytochemistry: suitable
• isotipo: IgG3
• Condiciones de envío: ambient
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... GALC(2581)

Descripción

Descripción general

Galactocerebroside (GalCer or GalC) is the most frequently found glycosphingolipid in animals. GalCer consist of a ceramide linked to a single galactose residue at the 1-hydroxyl moiety. Cerebrosides are concentrated in nervous tissues where they increase from about 0.02% of the dry weight in human fetal brain to 2% in gray matter and 12% in white matter of adult brain. GalCer is a specific cell-surface antigenic marker for oligodendrocytes and schwann cells. GalCer is the largest single component of the myelin sheath of nerves. GalCer synthesis therefore serves as a measurement of myelin formation or remyelination. Krabbe disease is an autosomal recessive sphingolipidosis caused by deficient activity of the lysosomal hydrolase galactosylceramide beta-galactosidase (GALC). GALC mediates the degradation of GalCer and other terminal beta-Gal-containing sphingolipids, including psychosine (galactosylsphingosine). Increased psychosine levels are believed to lead to widespread destruction of oligodendroglia in the CNS and to subsequent demyelination.

Especificidad

Galactocerebroside (GalC), sulfatide, psychosine and other galactolipids. Cross-reacts with the sulfatide ester of GalC, but to a 16-fold lesser extent. No cross-reactivity with sphingosine, ceramide, mixed ganglioside or glucocerebroside. Binds specifically to the surface of oligodendrocytes and Schwann cells (Ranscht, B. et al. (1982) Proc. Natl. Acad. Sci. U. S. A. 79(8):2709-2713).

Target lipid structure is not species-specific.

Inmunógeno

Synaptic plasma membranes from bovine hippocampus (Ranscht, B. et al. (1982) Proc. Natl. Acad. Sci. U. S. A. 79(8):2709-2713).

Aplicación

Anti-Galactocerebroside, clone mGalC, Alexa Fluor^™ 555 conjugate, Cat. No. MAB342-AF555, is a highly specific mouse monoclonal antibody conjugate that targets galactocerebroside (GalC) and has been tested in Immunocytochemistry.

Research Category
Neuroscience

The unconjugated antibody (Cat. No. MAB342) and other conjugates (Cat. Nos. FCMAB312F, MAB342-AF647 and MAB342A4) are also available for ELISA, Flow Cytometry, Immunocytochemistry, and Immunofluorescence applications.

Calidad

Evaluated by Immunocytochemistry in rat Schwann cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody immunostained non-permeabilzed rat schwann cells.

Forma física

Protein A purified.

Purified mouse IgG3 Alexa Fluor^™ 555 conjugate in PBS with 1.5% BSA and 0.09% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Información legal

ALEXA FLUOR is a trademark of Life Technologies

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable