MAB3100

Anti-Connexin 45 Antibody, near CT, cytoplasmic, clone 8A11.2

clone 8A11.2, Chemicon^®, from mouse

• Sinónimos: Cx45, Gap Junction gamma-1 protein, Gap Junction alpha-7 protein, Cx-45, GJA7 protein, GJC1 protein
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: 8A11.2, monoclonal
• reactividad de especies: dog (Wang, et al 2001), rabbit (Chaytor, et al 2005), chicken (Elenes, et al 2001), mouse, human, rat (Sorensen, et al 2008)
• fabricante / nombre comercial: Chemicon^®
• técnicas: immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotipo: IgG1
• Nº de acceso UniProt: P36383
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: dog ... Gjc1(490936) ; human ... GJC1(10052) ; mouse ... Gjc1(14615) ; rat ... Gjc1(266706)

Descripción

Descripción general

Connexin 45 is member of a family of gap junction proteins known as Connexin gene family. It is expressed in a wide variety of cell types during development and adult life, particularly in cardiac and smooth muscle tissues as well as brain tissues. Connexin 45 forms part of a network of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. There a number of alternatively spliced transcript variants that all seen to encode the same protein isoform.

Especificidad

Clone 8A11.2 is highly specific for Connexin 45 protein with no cross reactivity as analyzed by western blotting to any other connexin family member.

Others species not tested. Also confirmed to work on rat C6 cells.

Inmunógeno

Epitope: near C-terminus, cytoplasmic

The peptide sequence used for the immunogen was H-Gln-Ala-Tyr-Ser-His-Gln-Asn-Asn-Pro-His-Gly-Pro-Arg-Glu-Cys-OH conjugated to KLH, corresponding to amino acids 354-367 of human connexin 45.

Aplicación

Anti-Connexin 45 Antibody, near C-terminus, cytoplasmic, clone 8A11.2 is an antibody against Connexin 45 for use in IC, IH & WB.

Immunblotting (~46 kDa): 0.5 -1 µg/mL with ECL detection. Samples should be prepared with proteinase inhibitors and kept cold for best results. Alkali extraction methods can be used to enrich for connexin protein fractions. Briefly, 1 ml of NaHCO3 containing protease and phosphatase inhibitors and 22 µl of 1 M NaOH were added to frozen cell pellets. This cell suspension was sonicated for 30 s and incubated on ice for 50 min. Then, the homogenate was centrifuged for 30 min at 30,000 X g at 4°C. The supernatant was discarded, and the pellet was resuspended in sample buffer. {Elenes, S et al 2001}. 8% SDS_PAGE gels and PVDF membranes are suggested for greatest clarity.

Immunohistochemistry: fresh frozen, methanol fixed sections {Ujiie H et al (2003)}; 4% fixed frozen sections {Ikeda, Y et al 2007). 1-10 µg/mL with overnight incubations; 4% PFA fixed, paraffin-embedded sections {Gluhak-Heinrich, J et al 2007}, 1:50 with enzymatic detection.

Immunocytochemistry: 2% PFA fixed cells (15’, RT), permeabilized with a solution of 0.2% triton X-100/4% BSA {Sorensen et al 2008}.


Optimal working dilutions must be determined by end user.

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

Forma física

Format: Purified

Liquid at 1 mg/mL in 0.02M PB with 0.25M NaCl,pH=7.6 containing 0.1% sodium azide as a preservative.

Almacenamiento y estabilidad

Maintain at 2° to 8°C in undiluted aliquots for up to 12 months fromd date of receipt. Prevent multiple warming and coolings. Aliquot in tightly closed tubes to prevent loss.

Nota de análisis

Control
Cardiac tissue, particularly sinoatrial tissues.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable