AB1726
Anti-Connexin 40 Antibody
Chemicon®, from rabbit
Sinónimos:
Cx40, Gap Junction alpha-5 Protein, CxA-5
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
origen biológico
rabbit
Nivel de calidad
forma del anticuerpo
affinity isolated antibody
tipo de anticuerpo
primary antibodies
clon
polyclonal
purificado por
affinity chromatography
reactividad de especies
mouse, rat, human
fabricante / nombre comercial
Chemicon®
técnicas
immunohistochemistry: suitable
western blot: suitable
Nº de acceso NCBI
Nº de acceso UniProt
Condiciones de envío
wet ice
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... GJA5(2702)
Descripción general
Mouse Connexin 40 is a 358 amino acid gap junction protein with a predicted M.W. of ~40 kDa. It is prominently expressed in lung, heart and skin (see reviews: Kumar & Giula 1996; White, 1995; Evans, 1994; Beyer , 1990; Henneman, 1992; Traub, 1994; Goliger & Paul 1994). One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. A connexon is composed of a hexamer of connexins.
Especificidad
Reactivity with other species has not been determined.
Reacts with mouse connexin 40.
Inmunógeno
Anti-Connexin 40 is made against a 19 amino acid peptide sequence within the C-terminal cytoplasmic domain of mouse C×40 (2). Peptide sequence is conserved in human and rat Cx40 (100%), chicken Cx42 (77%), human Cx50 (87%), rat Cx50 (81%) and sheep Cx49 (87%).
Aplicación
Anti-Connexin 40 Antibody is an antibody against Cx40 for use in IHC & WB.
Immunohistochemistry Analysis: An 1:50 dilution of a representative lot detected Connexin 40 immunoreactivity associated with the gap junction between the cardiac myoepithial cells in mouse heart tissue sections.
Western Blot Analysis: An 1:1000 dilution of a representative lot detected Connexin 40 in human (HEK293, HeLa, HepG2, HUVEC, Jurkat) and murine (C2C12, HIN/3T3) cell lysates.
Western Blot Analysis: An 1:000 dilution of a representative lot detected Connexin 40 in human, mouse, and rat heart tissue homogenates, as well as in mouse brain tissue homogenates.
Optimal working dilutions must be determined by end user.
Western Blot Analysis: An 1:1000 dilution of a representative lot detected Connexin 40 in human (HEK293, HeLa, HepG2, HUVEC, Jurkat) and murine (C2C12, HIN/3T3) cell lysates.
Western Blot Analysis: An 1:000 dilution of a representative lot detected Connexin 40 in human, mouse, and rat heart tissue homogenates, as well as in mouse brain tissue homogenates.
Optimal working dilutions must be determined by end user.
Calidad
Evaluated by Western Blot on Jurkat lysates.
Western Blot Analysis: A 1:1000 dilution of this lot detected Connexin 40 in 10 μg of Jurkat lysates.
Western Blot Analysis: A 1:1000 dilution of this lot detected Connexin 40 in 10 μg of Jurkat lysates.
Descripción de destino
40 kDa
Forma física
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 15 mM NaCl containing 0.05% sodium azide.
Nota de análisis
Control
Placenta lysate.
Placenta lysate.
Otras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Información legal
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Volker J Schmidt et al.
PloS one, 8(11), e78782-e78782 (2013-11-16)
Gap junctions are involved in vascular growth and their expression pattern is modulated in response to hemodynamic conditions. They are clusters of intercellular channels formed by connexins (Cx) of which four subtypes are expressed in the cardiovascular system, namely Cx37
Connexin expression and conducted vasodilation along arteriolar endothelium in mouse skeletal muscle.
Looft-Wilson, Robin C, et al.
Journal of Applied Physiology, 97, 1152-1158 (2004)
Independence of connexin expression and vasomotor conduction from sympathetic innervation in hamster feed arteries.
Robin C Looft-Wilson, Sara J Haug, P Darrell Neufer, Steven S Segal
Microcirculation (New York, N.Y. : 1994) (1994)
Markus Boettcher et al.
Hypertension (Dallas, Tex. : 1979), 57(4), 802-808 (2011-03-02)
The endothelium-derived hyperpolarizing factor (EDHF) contributes critically to the regulation of vascular tone. Its dependency on direct signaling through myoendothelial gap junctions composed of connexins (Cx) is controversially discussed. We studied the impact of Cx40 in EDHF-type dilations in vivo
Reciprocal changes in endothelium-derived hyperpolarizing factor- and nitric oxide-system in the mesenteric artery of adult female rats following ovariectomy
Nawate, S. et al.
British Journal of Pharmacology, 144(2), 178-189 (2005)
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