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MAB1083

Sigma-Aldrich

Anti-GFP Antibody, clone 3F8.2

clone 3F8.2, from mouse

Sinónimos:

Green Fluorescent protein

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

forma del anticuerpo

purified immunoglobulin

tipo de anticuerpo

primary antibodies

clon

3F8.2, monoclonal

reactividad de especies

vertebrates

técnicas

western blot: suitable

isotipo

IgG1κ

Nº de acceso UniProt

Condiciones de envío

wet ice

modificación del objetivo postraduccional

unmodified

Descripción general

The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27 kDa monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light.

Especificidad

Reacts with most common vertebrate proteins containing the GFP tag.
The antibody reacts with the 28 kDa GFP protein.

Inmunógeno

Epitope: Full Length
Full length GFP protein.

Aplicación

Detect GFP using this Anti-GFP Antibody, clone 3F8.2 validated for use in WB.
Research Category
Secondary & Control Antibodies
Research Sub Category
Epitope Tags
Western Blot Analysis:
0.125 μg/mL - 1 μg/mL of this antibody detected GFP in 10 ng of GFP recombinant protein (Cat no. 14-392).

Calidad

Routinely evaluated by Western Blot on GFP recombinant protein.

Descripción de destino

28 kDa

Forma física

Format: Purified
Protein G Purified
Purified mouse immunoglobulin in buffer containing PBS containing 0.05% sodium azide.

Almacenamiento y estabilidad

Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Nota de análisis

Control
Western Blot: GFP recombinant protein

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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