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Merck
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Key Documents

IP05

Millipore

Protein G Plus/Protein A Agarose Suspension

Protein G PLUS/Protein A-Agarose mixture specifically formulated for immunoprecipitation.

Sinónimos:

Affinity resin, Protein G/Agarose

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About This Item

Código UNSPSC:
41116133
NACRES:
NA.56

formulario

slurry (Liquid)

contiene

≤0.1% sodium azide as preservative

fabricante / nombre comercial

Calbiochem®

condiciones de almacenamiento

do not freeze

técnicas

protein purification: suitable

idoneidad

suitable for microbiology

Condiciones de envío

wet ice

temp. de almacenamiento

2-8°C

Descripción general

Designed for immunoprecipitation applications. This product is blocked with BSA to reduce non-specific binding and cannot be used for purification.
Protein G PLUS/Protein A-Agarose mixture specifically formulated for immunoprecipitation.

Aplicación

Immunoprecipitation (see comments)

Advertencia

Toxicity: Standard Handling (A)

Forma física

33% slurry in PBS.

Otras notas

Agarose solution is supplied ready to use. Protein G Plus/Protein A Agarose immunoprecipitation reagent is blocked with BSA and should not be used for immunoglobulin purification or covalent cross-linking. For immunoprecipitation reactions 15 µl of solution per µg primary antibody is recommended. Preclearing will minimize extra bands resulting from nonspecific precipitation. To preclear, add to the sample 20 µl of agarose conjugate and 1 µg of normal IgG from the same species as the immunoprecipitating antibody. When immunoblotting is used for detection, some secondary antibodies can react nonspecifically with BSA or other proteins present at high concentrations in the sample. This can be eliminated by reducing the concentration of secondary antibody.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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