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Key Documents

FCMAB229F

Sigma-Aldrich

Milli-Mark® Mouse IgG1-k, clone MOPC-21, FITC conjugate

Detect mouse IgG1 using this Milli-Mark Anti-mouse IgG1-FITC Antibody, clone MOPC-21 validated for use in FC.

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

conjugado

FITC conjugate

forma del anticuerpo

purified immunoglobulin

clon

MOPC-21, monoclonal

reactividad de especies

human

fabricante / nombre comercial

Milli-Mark®

técnicas

flow cytometry: suitable

isotipo

IgG1κ

Condiciones de envío

wet ice

modificación del objetivo postraduccional

unmodified

Descripción general

The MOPC-21 tumor line that produces mouse IgG1,k, is a mineral oil induced plasmacytoma. The product is originated and carried intraperitoneally in BALB/c mice. Identity and purity of the immunoglobulin is established by immunoelectrophoresis (IEP) and Ouchterlony Double Diffusion (ODD), prior to conjugation. Electrophoresis of the purified immunoglobulin, followed by diffusion versus anti-mouse whole serum, antimouse IgG1 and anti-mouse kappa chain, results in single arcs of precipitation. By ODD the purified immunoglobulin preparation is non-reactive with antisera to mouse lambda light chain, mouse IgA, IgM, IgG2a, IgG2b, and IgG3. The specificity of staining by monoclonal antibodies to human CD antigens should be verified by establishing the amount of non-specific binding to the target cell population. It is recommended that a non-reactive immunoglobulin of the same isotype, concentration and F/P molar ratio be included as a negative control for each monoclonal antibody reagent used in flow cytometry or other immunoassays.

Especificidad

This mouse IgG1 isotype control antibody is not reactive with any known antigen.

Aplicación

Research Category
Inflammation & Immunology
Research Sub Category
Isotype Control Antibodies

Calidad

Evaluated for use as flow cytometry isotype control using Jurkat Cells.

Forma física

Immunoelectrophoresis
Purified mouse monoclonal IgG1κ conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Almacenamiento y estabilidad

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Información legal

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

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A Barbonetti et al.
Biology of reproduction, 79(4), 649-656 (2008-06-20)
Phosphorylation of tyrosine residues in cellular proteins represents a major event during sperm capacitaton, but its relationship with the acquisition of sperm-fertilizing ability is still unclear. In this study we explored the relationship between the kinetics of the global tyrosine

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