EZRMFGF21

Rat/Mouse FGF-21 ELISA Kit

measures and quantifies FGF-21 levels in 10 µl - 20 µl serum, plasma or cell culture supernatent

• Sinónimos: Fibroblast growth factor 21
• Código UNSPSC: 12161503
• eCl@ss: 32161000
• NACRES: NA.32

Propiedades

• Nombre del producto: Rat / Mouse FGF-21 ELISA Kit, This Rat / Mouse FGF-21 ELISA Kit is used to measure & quantify FGF-21 levels in Metabolism & Inflammation & Immunology research.
• Nivel de calidad: 200
• reactividad de especies: mouse, rat
• envase: kit of 1 × 96 wells
• Parámetros: 10—20 μL sample volume (4hr assay)
• assay range: standard curve range: 50-12000 pg/mL; inter-assay cv: <8%; intra-assay cv: <10%
• técnicas: ELISA: suitable
• entrada: sample type cell culture supernatant; sample type serum; sample type plasma (K2 EDTA)
• aplicaciones: research use
• método de detección: colorimetric (450nm/590nm)
• Condiciones de envío: wet ice
• temp. de almacenamiento: 2-8°C

Descripción

Descripción general

Recent studies have demonstrated that fibroblast growth factor 21 (FGF-21), a liver derived protein, is able to increase glucose uptake, as well as lower plasma triglyceride levels in an insulin-independent manner. FGF-21 suppresses glucagon release, thereby increasing insulin sensitivity, and reduces the amount of insulin production by pancreatic β-cells. Unlike insulin, which works in a rapid, hormone-like manner, FGF-21 activity requires several hours, indicating changes in gene expression. No significant weight gain was observed when FGF-21 was injected into animals, and mice that had been genetically engineered to overexpress FGF-21 weighed less and had less adipose tissue than the normal controls.

Although considerable progress has been made in the understanding of the pathobiology of type 2 diabetes, as well in the development of new compounds for the treatment of diabetic patients, this disease remains a major global health concern. Consequently, Millipore is pleased to offer the Rat/Mouse FGF-21 ELISA kit for the quantification of FGF-21 in rat and mouse serum and plasma samples. The insulin-independent activities of FGF-21, as well as the absence of the weight gain effects of insulin, thiazolidinediones and other anti-diabetic drugs, distinguish it from current insulin-sensitizing drugs, making it a promising therapeutic.

Especificidad

Cross-reactivity to Human FGF-21: 39%

No significant cross-reactivity to the cytokines and metabolic hormones tested was observed.

Aplicación

Research Category
Metabolism

Research Sub Category
Diabetes - Type 2

Metabolic Syndrome

This is a 3-hour assay requiring 20 μL (in duplicate) of rat sample or 10µl (in duplicate) of mouse sample.

Used to detect/quantify: FGF-21

Otras notas

Please contact Technical Service for linearity of dilution.

Cláusula de descargo de responsabilidad

For research use only. Not for use in diagnostic procedures.

Información de seguridad

• Pictogramas: GHS06,GHS05,GHS09
• Palabra de señalización: Danger
• Frases de peligro: H290,H302 + H332,H311,H317,H319,H335,H411
• Consejos de prudencia: P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338
• Clasificaciones de peligro: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Met. Corr. 1 - Skin Sens. 1 - STOT SE 3
• Órganos de actuación: Respiratory system
• Código de clase de almacenamiento: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects