CBL188
Anti-Clathrin Antibody, clone CHC5.9
clone CHC5.9, Chemicon®, from mouse
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
origen biológico
mouse
Nivel de calidad
forma del anticuerpo
purified antibody
tipo de anticuerpo
primary antibodies
clon
CHC5.9, monoclonal
reactividad de especies
bovine, amphibian, rat, pig, human
fabricante / nombre comercial
Chemicon®
técnicas
immunohistochemistry: suitable
western blot: suitable
isotipo
IgM
Nº de acceso NCBI
Nº de acceso UniProt
Condiciones de envío
wet ice
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... CLTC(1213)
Especificidad
This antibody reacts with the clathrin heavy chain from a diverse range of species. In nervous tissue it cross-reacts with another coated vesicle polypeptide of 190 kDa which is not present in isolated clathrin triskelions. In some epithelial cells of certain species the antibody may cross-react with a cytokeratin component. It detects coated vesicles from different organs and tissues (e.g. brain, ovary, mammary gland). : Recognizes bovine, rat and amphibian
Inmunógeno
Coated vesicles (clathrin) of bovine brain.
Aplicación
Anti-Clathrin Antibody, clone CHC5.9 is an antibody against Clathrin for use in WB, IH.
Marker for detection of receptor mediated endocytosis
Suitable for frozen sections
Western blotting KNOW SPECIES
Optimal working dilutions must be determined by the end user.
Suitable for frozen sections
Western blotting KNOW SPECIES
Optimal working dilutions must be determined by the end user.
Research Category
Epitope Tags & General Use
Epitope Tags & General Use
Research Sub Category
Organelle & Cell Markers
Organelle & Cell Markers
Forma física
Format: Purified
The antibody was purified by gel filtration and is presented as 100μg of lyophilysed material. Reconstitute with 1ml distilled water. Final buffer composition is PBS, pH 7.4.
Almacenamiento y estabilidad
Maintain at 2-8°C for up to 12 months from date of receipt.
Nota de análisis
Control
Positive reactions using HeLa, SV-40 and RVF-SMC cultured cell lines.
Positive reactions using HeLa, SV-40 and RVF-SMC cultured cell lines.
Información legal
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de clase de almacenamiento
13 - Non Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Hisako Furukawa et al.
Laboratory investigation; a journal of technical methods and pathology, 84(7), 828-835 (2004-05-04)
Prion protein (PrP) is known to accumulate in some non-neuronal tissues under conditions unrelated to prion diseases. The biochemical and biological nature of such accumulated PrP molecules, however, has not been fully evaluated. In this study, we established experimental myopathy
Molecular links between endocytosis and the actin cytoskeleton.
B Qualmann et al.
The Journal of cell biology, 150(5), F111-F116 (2000-09-07)
J Hirst et al.
Biochimica et biophysica acta, 1404(1-2), 173-193 (1998-08-26)
Clathrin and adaptors are components of clathrin-coated pits and vesicles. The AP-1 adaptor complex is associated with clathrin-coated vesicles budding from the TGN, while the AP-2 adaptor complex is associated with clathrin-coated vesicles budding from the plasma membrane. The clathrin
G Bruder et al.
Experimental cell research, 164(2), 449-462 (1986-06-01)
We have used a monoclonal antibody (CHC5.9) to identify clathrin (Mr 180,000; 'heavy chain') in coated vesicles, triskelion structures prepared in vitro and in high-speed supernatants (HSS) of cell homogenates from a variety of tissues and species (e.g., brain and
Nadezhda V Popova et al.
Journal of neurochemistry, 118(6), 988-998 (2011-07-14)
TPR-containing Rab8b-interacting protein (TRIP8b) is a brain-specific hydrophilic cytosolic protein that contains tetratricopeptide repeats (TPRs). Previous studies revealed interaction of this protein via its TPR-containing domain with Rab8b small GTPase, hyperpolarization-activated cyclic nucleotide-regulated channel (HCN) channels and G protein-coupled receptor
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