ABN99

Anti-phospho NR1 (Ser897) Antibody

from rabbit, purified by affinity chromatography

• Sinónimos: glutamate receptor, ionotropic, N-methyl D-aspartate 1, N-methyl-D-aspartate receptor subunit NR1, glutamate [NMDA] receptor subunit zeta 1, N-methyl-D-aspartate receptor channel, subunit zeta-1, glutamate [NMDA] receptor subunit zeta-1
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: rat
• reactividad de especies (predicha por homología): mouse (based on 100% sequence homology), human (based on 100% sequence homology)
• técnicas: immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Nº de acceso UniProt: Q05586
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: phosphorylation (pSer897)
• Información sobre el gen: human ... GRIN1(2902) ; mouse ... Grin1(14810) ; rat ... Grin1(24408)

Descripción

Descripción general

NR1 (NMDA1or NMDAR1, also known as GRIN1) along with the NR2 subunit forms a heterodimer that make up the NMDA receptor. The NMDA receptor (NMDAR) is characterized as a ionotropic receptor for glutamate. As the name suggests, (NMDA (N-methyl D-aspartate) is an agonist for the receptor. A distinct characteristic of the NMDA receptor is that it is both ligand-gated and voltage-dependent based on its sensitivity to magnesium. Activated NMDA receptors result in the opening of an ion channel that is nonselective to cations. The result is the flow of Na+ and small amounts of Ca2+ ions into the cell and K+ out of the cell. Calcium flux by the NMDARs has been implicated in synaptic plasticity, a mechanism in the cell for learning and memory.

Especificidad

This antibody recognizes NR1 when phosphorylated at Ser897.

Inmunógeno

Epitope: Phosphorylated Ser897

KLH-conjugated linear peptide corresponding to rat NR1 when phosphorylated at Ser897.

Aplicación

Immunohistochemistry Analysis: 1:50 dilution from a previous lot detected NR1 in normal rat hippocampus.
Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated NR1 from rat brain microsomal RIPA lysate.
Western Blot Analysis: 2 µg/mL from a previous lot detected NR1 in Lambda phosphatase untreated and treated rat brain microsomal tissue lysate.

Research Category
Neuroscience

Research Sub Category
Signaling Neuroscience

This Anti-phospho NR1 (Ser897) Antibody is validated for use in WB, IP, IH for the detection of phospho NR1 (Ser897).

Calidad

Evaluated by Western Blot in rat brain microsomal tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected NR1 on 10 µg of rat brain microsomal tissue lysate.

Descripción de destino

~ 120 kDa

Ligadura / enlace

Replaces: 06-641

Forma física

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Nota de análisis

Control
Rat brain microsomal tissue lysate

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable