ABE415

Anti-Transcription factor EB Antibody

from rabbit, purified by affinity chromatography

• Sinónimos: Transcripiton factor EB, Class E basic helix-loop-helix protein 35, bHLHe35
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: human
• reactividad de especies (predicha por homología): chimpanzee (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology), baboon (based on 100% sequence homology), orangutan (based on 100% sequence homology), gibbon (based on 100% sequence homology)
• técnicas: immunoprecipitation (IP): suitable; western blot: suitable
• Nº de acceso NCBI: NP_009093
• Nº de acceso UniProt: P19484
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... TFEB(7942)

Descripción

Descripción general

The protein named Transcription factor EB (TFEB) or alternatively in older literature, Class E basic helix-loop-helix protein 35 (bHLHe35) and encoded by the human gene TFEB/BHLH35 is a transcription factor that specifically recognizes and binds E-box sequences (5′-CANNTG-3′) on DNA. Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. TFEB in association with the protein TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4+ T-cells and thymus-dependent humoral immunity. TFEB also plays a central role in expression of lysosomal genes. TFEB specifically recognizes and binds the CLEAR-box sequence (5′-GTCACGTGAC-3′) present in the regulatory region of many lysosomal genes and through binding acts as a positive regulator of their expression. Finally, TFEB appears to be a positive regulator for autophagy related gene expression, heavy chain immunoglobulin gene expression, and genes necessary for the proper vascularization of important tissues such as the placenta.

Inmunógeno

KLH-conjugated linear peptide corresponding to a region near the C-terminus of human Transcription factor EB.

Aplicación

Detect TFEB using this rabbit polyclonal antibody, Anti-Transcription factor EB Antibody validated for use in western blotting & IP.

Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated Transcription factor EB in 500 µg of Raji cell lysate. Immunopecipitated Transcription factor EB was then detected in Western Blot.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Calidad

Evaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Transcription factor EB in 10 µg of Raji cell lysate.

Descripción de destino

~58 kDa and ~40 kDa observed. Uniprot describes two isoforms at 44 kDa and 53 kDa This protein has been observed at 44-53 kDa due to sumoylation (Miller, A. J., et al. (2005). J Biol Chem. 280(1):146-55.).

Forma física

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable