AB5612
Anti-Citrulline Antibody
serum, Chemicon®
Sinónimos:
CCP
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
origen biológico
rabbit
Nivel de calidad
forma del anticuerpo
serum
tipo de anticuerpo
primary antibodies
clon
polyclonal
reactividad de especies (predicha por homología)
mammals
fabricante / nombre comercial
Chemicon®
técnicas
ELISA: suitable
immunohistochemistry: suitable
idoneidad
not suitable for immunohistochemistry (Paraffin)
Condiciones de envío
wet ice
modificación del objetivo postraduccional
unmodified
Especificidad
Citrulline in glutaraldehyde fixed tissues or cells.
The cross-reactivities were determined using an ELISA test by competition experiments with the following compounds:
Compound Cross-reactivity ratio
Citrulline-G-BSA 1
Arginine-G-BSA 1/>100,000
Glutamine-G-BSA 1/>100,000
Ornithine-G-BSA 1/>100,000
Abbreviations:
(G) Glutaraldehyde
(BSA) Bovine Serum Albumin
The cross-reactivities were determined using an ELISA test by competition experiments with the following compounds:
Compound Cross-reactivity ratio
Citrulline-G-BSA 1
Arginine-G-BSA 1/>100,000
Glutamine-G-BSA 1/>100,000
Ornithine-G-BSA 1/>100,000
Abbreviations:
(G) Glutaraldehyde
(BSA) Bovine Serum Albumin
Inmunógeno
Citrulline-Glutaraldehyde-BSA
Aplicación
Anti-Citrulline Antibody is a Rabbit Polyclonal Antibody for the detection of Citrulline & has been validated in ELISA & IHC.
Immunohistochemistry: 1:1,000-1:2,500 glutaraldehyde required for reactivity. (0.5%-2.5% is typical; fluorescence detection is not recommended. Blocking is with 3% normal serum with 0.1-0.2% triton X-100 usually. Primary antibody is diluted only in TBS or PBS with out blocking or triton X-100. Incubations are usually 3 hours 37C or 4C overnight. Detection is by ABC or PAP for best results. Not recommended for paraffin embedded tissues.
ELISA: 1:2500-1:5000
Optimal working dilutions must be determined by the end user.
ELISA: 1:2500-1:5000
Optimal working dilutions must be determined by the end user.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Chromatin Biology
Forma física
Rabbit antiserum. Liquid with 0.05% sodium azide
Almacenamiento y estabilidad
Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
Información legal
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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The periodontium of periodontitis patients contains citrullinated proteins which may play a role in ACPA (anti-citrullinated protein antibody) formation.
Willem Nesse,Johanna Westra,Jacqueline E van der Wal,Frank Abbas,Anthony P Nicholas et al.
Journal of Clinical Periodontology null
Nitric oxide modulates peristaltic muscle activity associated with fluid circulation in the sea pansy Renilla koellikeri.
Anctil, M; Poulain, I; Pelletier, C
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Charlotte Thålin et al.
Journal of thrombosis and haemostasis : JTH, 18(10), 2732-2743 (2020-07-13)
Recent data propose a diagnostic and prognostic capacity for citrullinated histone H3 (H3Cit), a marker of neutrophil extracellular traps (NETs), in pathologic conditions such as cancer and thrombosis. However, current research is hampered by lack of standardized assays. We aimed
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