AB5174
Anti-Potassium Channel Kv1.1 Antibody
Chemicon®, from rabbit
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
origen biológico
rabbit
Nivel de calidad
forma del anticuerpo
affinity purified immunoglobulin
tipo de anticuerpo
primary antibodies
clon
polyclonal
purificado por
affinity chromatography
reactividad de especies
mouse, rat
fabricante / nombre comercial
Chemicon®
técnicas
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Nº de acceso NCBI
Nº de acceso UniProt
Condiciones de envío
dry ice
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... KCNA1(3736)
Especificidad
Recognizes a full length Kv1.1 protein. Does not cross react with any other potassium channel antigens tested so far.
Inmunógeno
GST fusion protein and a C-terminal portion of mouse Kv1.1 protein (amino acids 416-495) (Accession P16388).
Aplicación
Anti-Potassium Channel Kv1.1 Antibody detects level of Potassium Channel Kv1.1 & has been published & validated for use in IH, IP & WB.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Ion Channels & Transporters
Ion Channels & Transporters
Western blot: 1:200 using ECL; detects ~ 86kDa band on mouse membranes; some smaller minor bands may also be present. Mature channels are glycosylasted which can lead to smeary or slightly larger bands in certain preparations. It is important to use a full range of proteinase inhbitors to obtain full length protein blots.
Immunohistochemistry on rat brain sections.
Immunoprecipitation
Dilutions should be made using a carrier protein such as BSA (1-3%)
Optimal working dilutions must be determined by the end user.
Immunohistochemistry on rat brain sections.
Immunoprecipitation
Dilutions should be made using a carrier protein such as BSA (1-3%)
Optimal working dilutions must be determined by the end user.
Forma física
Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.025% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
Almacenamiento y estabilidad
Maintain lyophilized material at -20°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Nota de análisis
Control
CONTROL ANTIGEN: Included free of charge with the antibody is 120 μg of control antigen (lyophilized powder in phosphate buffered saline, pH 7.4, containing 5% sucrose and 0.025% sodium azide). The stock solution of the antigen can be made up using 200 μL of sterile deionized water. For positive control, in Western blot using 40 ng of protein per lane. For negative control, preincubate 3-5 μg of fusion protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
CONTROL ANTIGEN: Included free of charge with the antibody is 120 μg of control antigen (lyophilized powder in phosphate buffered saline, pH 7.4, containing 5% sucrose and 0.025% sodium azide). The stock solution of the antigen can be made up using 200 μL of sterile deionized water. For positive control, in Western blot using 40 ng of protein per lane. For negative control, preincubate 3-5 μg of fusion protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Otras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Información legal
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Aquatic Chronic 3
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Testing null
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