AB4332

Anti-acetyl-MVH (Lys405) Antibody

from rabbit, purified by affinity chromatography

• Sinónimos: Probable ATP-dependent RNA helicase DDX4, DEAD box protein 4, Mvh, Vasa homolog
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: pig, human, mouse
• reactividad de especies (predicha por homología): porcine (based on 100% sequence homology), rat (based on 100% sequence homology)
• técnicas: dot blot: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable; inhibition assay: suitable (peptide); western blot: suitable
• Nº de acceso NCBI: NP_034159
• Nº de acceso UniProt: Q61496
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: acetylation (Lys405)
• Información sobre el gen: mouse ... Ddx4(13206)

Descripción

Descripción general

MVH (also known as DDX4 or Vasa) is a member of the DEAD family of proteins and is a probable ATP-dependent RNA helicase. MVH is mostly localized in testicular germ cells in the cytoplasm of spermatocytes. MVH is a high-copy extragenic suppressor gene. MVH is shown to be significantly decreased in spermatozoa of men that are infertile and may have an important role in male fertility and in stem cell development.

Especificidad

This antibody specifically recognizes wild type MVH acetylated at Lys405, but not the Lys405 mutant.

Inmunógeno

Epitope: Acetylated Lys405

KLH-conjugated linear peptide corresponding to mouse MVH acetylated at Lys405.

Aplicación

Anti-acetyl-MVH (Lys405) Antibody is a Rabbit Polyclonal Antibody for detection of acetyl-MVH (Lys405) also known as Probable ATP-dependent RNA helicase DDX4, DEAD box protein & has been validated in DB, WB, ICC, PIA & IP.

Research Category
Stem Cell Research

Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Western Blot/Immunoprecipitation Analysis: A representative lot detected Flag-tagged acetyl-MVH (Lys405) in the presence of Myc-HAT/p46.

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected acetyl-MVH (Lys405) in mouse germ cells (I. Nagamori, Department of Pharmacology, University of California, Irvine).

Calidad

Evaluated by Dot Blot/Peptide Inhibition Assay in non-acetylated and acetylated MVH (Lys405).

Dot Blot/Peptide Inhibition Analysis: A 1:1,000 dilution of this antibody detected acetyl-MVH (Lys405) peptide.

Descripción de destino

76 kDa calculated

Forma física

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Nota de análisis

Control
Non-acetylated and acetylated MVH (Lys405)

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids