AB3098

Anti-Orexin-A Antibody

Chemicon^®, from rabbit

• Sinónimos: Anti-NRCLP1, Anti-OX, Anti-PPOX
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: affinity purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: feline, rat, human, mouse
• fabricante / nombre comercial: Chemicon^®
• técnicas: ELISA: suitable; western blot: suitable
• Nº de acceso NCBI: NM_001524.1
• Nº de acceso UniProt: O43612
• Condiciones de envío: dry ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... PPOX(5498)

Descripción

Especificidad

Mouse Orexin-A. Several peptides associated with feeding behavior have been reported recently. Orexins (Orexin-A and Orexin-B) are a family of hypothalamic neuropeptides selectively expressed in the hypothalamus (Sakurai et al. 1998). Orexin-A and Orexin-B are derived from the same precursor (Prepro-orexin) by proteolytic cleavage. Prepro-orexin is an 130 aa peptide with a putative 33 aa secretory sequence, a hydrophobic core followed by residues with small polar side chains. The expression was detected in brain and to a small extent in testis (Sakurai et al. 1998). These neuropeptides bind and activate two closely related Orexin receptors--G protein coupled receptors (GPCRs) OX1R and OX2R.

Inmunógeno

An 18 amino acid peptide mapping near the amino terminus of mouse Orexin-A. The immunogen sequence is identical in human, rat, porcine, ovine, bovine and canine and has 81% homology with chicken.

Aplicación

Research Category
Neuroscience

Research Sub Category
CNS Control of Metabolism

Circadian Rhythm & Sleep

This Anti-Orexin-A Antibody is validated for use in ELISA, WB for the detection of Orexin-A.

Western blot: 1-10 μg/mL using Chemiluminescence technique ELISA: 1:10,000 - 100,000 using 5-100 ng of control peptide per well. NOTE: Orexin A is a 3.5kDa protein. Adjust your SDS-PAGE gel and lane size markers accordingly.

Immunohistochemistry: Frozen 4% PFA tissue with ABC detection {Wagner et al 2000}.

Optimal working dilutions must be determined by end user.

Forma física

Liquid in PBS, pH 7.4 with 0.1% BSA and 0.1% sodium azide.

Almacenamiento y estabilidad

Maintain frozen at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable