AB3092

Anti-Orexin-1 Receptor Antibody

Chemicon^®, from rabbit

• Sinónimos: OX1R, Hypocretin Receptor 1
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: affinity purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: rat, human, mouse
• fabricante / nombre comercial: Chemicon^®
• técnicas: ELISA: suitable; western blot: suitable
• Nº de acceso NCBI: NM_001525.1
• Nº de acceso UniProt: O43613
• Condiciones de envío: dry ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... HCRTR1(3061)

Descripción

Descripción general

Several peptides associated with feeding behavior have been reported recently. Orexins (Orexin-A and Orexin-B) are a family of hypothalamic neuropeptides selectively expressed in the hypothalamus (Sakurai et al. 1998). Orexin-A and Orexin-B are derived from the same precursor (Prepro-orexin) by proteolytic cleavage. Prepro-orexin is an 130 aa peptide with a putative 33 aa secretory sequence, a hydrophobic core followed by residues with small polar side chains. The expression was detected in brain and to a small extent in testis (Sakurai et al. 1998). These neuropeptides bind and activate two closely related Orexin receptors--G protein coupled receptors (GPCRs) OX1R and OX2R.

Especificidad

Recognizes Rat and Human Orexin-1.

Inmunógeno

A 16 aa peptide near the N-terminus of rat OX1R and a 16 aa peptide near the C-terminus of human OX1R.

Aplicación

Research Category
Neuroscience

Research Sub Category
CNS Control of Metabolism

Circadian Rhythm & Sleep

This Anti-Orexin-1 Receptor Antibody is validated for use in ELISA, WB for the detection of Orexin-1 Receptor.

Western blot: 1-10 μg/mL using Chemiluminescence technique. Protein runs at 45-48 kDa.

ELISA: 1:10,000 -1:100,000 against 50-100 ng/wel immunogen peptide.

Optimal working dilutions must be determined by end user.

Descripción de destino

45-48 kDa

Forma física

Affinity Purified immunoglobulin. Liquid in PBS with 0.1% BSA.

ImmunoAffinity Purified

Almacenamiento y estabilidad

Maintain for 6 months at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Nota de análisis

Control
Hypothalamus

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable