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AB1964

Sigma-Aldrich

Anti-N-WASP Antibody, phospho-specific [Ser484/Ser485]

Chemicon®, from rabbit

Sinónimos:

Neural Wiskott-Aldrich Syndrome Protein

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

clon:
polyclonal
application:
ELISA
WB
reactividad de especies:
human, rat
técnicas:
ELISA: suitable
western blot: suitable
citations:
3
En este momento no podemos mostrarle ni los precios ni la disponibilidad

origen biológico

rabbit

Nivel de calidad

forma del anticuerpo

affinity isolated antibody

tipo de anticuerpo

primary antibodies

clon

polyclonal

purificado por

affinity chromatography

reactividad de especies

human, rat

fabricante / nombre comercial

Chemicon®

técnicas

ELISA: suitable
western blot: suitable

isotipo

IgG

Descripción general

Members of the Wiskott-Aldrich sydrome protein (WASP) family regulate the formation of actin-based cell structures in many cell types. These proteins contain C-terminal actin-binding domains that can stimulate actin polymerization. In addition, these proteins bind the ARP2/3 complex, which can nucleate actin polymerization at sites that lead to branched actin structures. WASP is expressed primarily in hematopoietic cells, while its homolog N-WASP is widely expressed. These proteins have 48% identity in human with the highest homology in the functional regions of these proteins. Phosphorylation regulates the activity of both proteins. Dual phosphorylation of WASP on serine 483 and 484 by casein kinases increase the affinity for the ARP2/3 complex. Thus, dual serine phosphorylation may be important for formation of actin-based structures in various cell types.

Especificidad

The antibody detects a 63 kDa protein corresponding to the molecular weight of phosphorylated WASP on SDS-PAGE immunoblots of Jurkat cell lysate. In rat brain, A431, human endothelial and SKN-SH cells, this antibody detects a 65 kDa protein corresponding to phosphorylated N-WASP.

Inmunógeno

Epitope: phosphoserine484/phosphserine485
Phospho-N-WASP (S484/S485) synthetic peptide (coupled to KLH) corresponding to amino acid residues around serine 484 and 485 of human N-WASP. The human WASP sequence has a similar peptide sequence surrounding serine 483 and 484.

Aplicación

Detect N-WASP using this Anti-N-WASP Antibody, phospho-specific [Ser484/Ser485] validated for use in ELISA & WB.
Research Category
Signaling
Research Sub Category
Cytoskeletal Signaling
Western blot: 1:1000 ELISA: 1:2000

Forma física

This antibody was cross-adsorbed to unphosphorylated-N-WASP (S484/S485) peptide (without carrier) then affinity purified using phospho-N-WASP (S484/S485) peptide (without carrier). Antibody is supplied in 100 μl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.

Almacenamiento y estabilidad

Maintain at 20°C for up to one year from date of receipt. Do not aliquot.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 2


Certificados de análisis (COA)

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Visite la Librería de documentos

Estrogen receptor-alpha promotes breast cancer cell motility and invasion via focal adhesion kinase and N-WASP.
Sanchez, AM; Flamini, MI; Baldacci, C; Goglia, L; Genazzani, AR; Simoncini, T
Molecular Endocrinology null
P Raaj Khusial et al.
Oncotarget, 1(3), 198-209 (2011-02-09)
Cell migration is an essential step in cancer invasion and metastasis. A number of orchestrated cellular events involving tyrosine kinases and signaling receptors enable cancer cells to dislodge from primary tumors and colonize elsewhere in the body. For example, activation

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