AB15332

Anti-Nicotinic Acetylcholine Receptor α7 Antibody

Chemicon^®, from rabbit

• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: rat, mouse, human
• fabricante / nombre comercial: Chemicon^®
• técnicas: western blot: suitable
• Nº de acceso NCBI: NM_000746.3
• Nº de acceso UniProt: P36544
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: mouse ... Chrna7(11441)

Descripción

Especificidad

This rabbit polyclonal antibody specifically detects Nicotinic Receptor 7. It targets an epitope with 28 amino acids from the C-terminal half.

Inmunógeno

KLH-conjugated linear peptide corresponding to 28 amino acids from the C-terminal half of human Nicotinic Receptor 7.

Aplicación

Quality Control Testing

Evaluated by Western Blotting in Rat brain tissue lysate.Western Blotting Analysis: A 1:500 dilution of this antibody detected Nicotinic Acetylcholine Receptor 7 in Rat brain tissue lysate.

Descripción de destino

Neuronal acetylcholine receptor subunit alpha-7 (UniProt: P36544; also known as -Nicotinic Receptor Alpha 7, Nicotinic AChR alpha7) is encoded by the CHRNA7 (also known as NACHRA7) gene (Gene ID: 1139) in human. Nicotinic acetylcholine receptors are cation-selective, ligand-gated ion channels that mediate fast neurotransmission. They are distributed at the post-synaptic membrane of muscle cells. A similar neuronal-type ACh receptor is also found in the central nervous system and is involved in a wide range of physiological and pathological functions. They are assembled either as homo-pentamers of 7, 8, and 9 or hetero-pentamers of 2 6 in combination with β2 β4 or 9 with 10 subunits. They regulate the flow of sodium, potassium, and calcium ions across the cell membrane. The binding of ligands to these receptors triggers a tertiary conformational transition of neuronal AChRs among functionally distinct resting, open, and desensitized states. Nicotinic AChR 7 is a multi-pass, homopentameric, membrane glycoprotein that is synthesized with a signal peptide (aa 1-22), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 23-230), four transmembrane domains (aa 231-255; 262-280; 296-317; and 470-490), and one cytoplasmic domain (aa 318-469). (Ref.: Ho, TN., et al. (2020). Front. Neurosci. 14; 609005; Unwin, N. (2013). Q. Rev. Biophys. 46(4); 283-322).

Ligadura / enlace

Replaces: AB5637

Forma física

Purified rabbit polyclonal antibody in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, and 0.1% sodium azide.

Almacenamiento y estabilidad

Recommended storage: +2°C to +8°C.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable