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Key Documents

AB1528

Sigma-Aldrich

Anti-Nerve Growth Factor-β Antibody

serum, Chemicon®

Sinónimos:

NGFbeta

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

sheep

Nivel de calidad

forma del anticuerpo

serum

tipo de anticuerpo

primary antibodies

clon

polyclonal

reactividad de especies

rat, human, chicken, mouse

no debe reaccionar con

bovine

fabricante / nombre comercial

Chemicon®

técnicas

ELISA: suitable
immunohistochemistry: suitable
western blot: suitable

Nº de acceso NCBI

Nº de acceso UniProt

Condiciones de envío

dry ice

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... NGF(4803)

Especificidad

Nerve growth factor-beta (NGF). Less than 1% cross-reactivity against recombinant human NT4 and BDNF and approximately 2% for NT3 by ELISA.

Inmunógeno

Mouse nerve growth factor-beta purified from salivary glands.

Aplicación

Detect Nerve Growth Factor-β using this Anti-Nerve Growth Factor-β Antibody validated for use in ELISA, WB, IH.
Immunohistochemistry at 1:500-1:5,000 (see suggested protocol).

Immunoblotting at 1:500-1:5,000.

ELISA at 1:500-1:5,000.

Inhibition of biological activity in vitro at 1:10-1:50.
BIOLOGICAL ACTIVITY: Neutralizes NGF, but not other neurotrophins.

Use neat for in vivo animal studies (5-10 μL/g body weight).

Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Research Sub Category
Neurochemistry & Neurotrophins

Forma física

Whole sheep antisera. Lyophilized. Reconstitute with 250 μL of sterile distilled water.

Almacenamiento y estabilidad

Maintain at -20°C to -70°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Hiroyuki Murota et al.
The Journal of allergy and clinical immunology, 130(3), 671-682 (2012-07-10)
Itch impairs the quality of life for many patients with dermatoses, especially atopic dermatitis (AD), and is frequently induced by a warm environment. To determine the mechanism underlying itch induction by warmth, we focused on artemin, a member of glial
D Joelson et al.
Microscopy research and technique, 41(3), 246-262 (1998-05-30)
The distribution of immunoreactivity for the ionotropic N-methyl-D-aspartate (NMDA) receptor subunits was mapped in the cochlear nucleus of postnatal day (P) 7, P14, P21, and P28 gerbils. Frozen sections and serial plastic sections of tissue were incubated with antibodies to
Daniel Aridgides et al.
PloS one, 8(2), e57450-e57450 (2013-02-26)
Cardiomyocytes express neurotrophin receptor TrkA that promotes survival following nerve growth factor (NGF) ligation. Whether TrkA also resides in cardiac fibroblasts (CFs) and underlies cardioprotection is unknown. To test whether CFs express TrkA that conveys paracrine signals to neighbor cardiomyocytes
X F Zhou et al.
Journal of neuroscience methods, 54(1), 95-102 (1994-09-01)
Nerve growth factor (NGF) is a survival factor required by a number of neuronal populations including most post-ganglionic sympathetic neurones. NGF has been detected and quantified in many tissues but there is little information regarding its cellular localization. Although it
I K Abrahamson et al.
Journal of neurocytology, 16(3), 417-422 (1987-06-01)
Immunohistochemistry has been used to demonstrate the presence of nerve growth factor (NGF)-like immunoreactivity in normal and sectioned mouse sciatic nerves. In normal nerves, immunoreactive material was not visible unless a silk ligature had previously been applied to constrict the

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