AB10029

Anti-Ubiquityl Histone H2A.X (Lys119) Antibody

serum, from rabbit

• Sinónimos: H2AXK119Ub, Histone H2A.X Ubiquityl K119), H2A histone family, member X, H2AX histone
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: serum
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• reactividad de especies: mouse, human
• reactividad de especies (predicha por homología): rat (based on 100% sequence homology), bovine (based on 100% sequence homology), canine (based on 100% sequence homology)
• técnicas: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Nº de acceso NCBI: NP_002096.1
• Nº de acceso UniProt: P16104
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: ubiquitination (Lys119)
• Información sobre el gen: bovine ... H2Ax(531733) ; dog ... H2Ax(489372) ; human ... H2AX(3014) ; mouse ... H2Ax(15270) ; rat ... H2Ax(500987)

Descripción

Descripción general

H2AX is involved in DNA repair and the maintenance of genomic stability. It is been implicated both in homologous recombination and nonhomologous end joining DNA repair pathways. H2AX is thought to have a critical function in the recruitment of DNA repair factors and DNA damage-signaling proteins. H2A.X is ubiquitinated by RING1 and RNF2/RING2 complex providing a specific mark for transcriptional repression. In addition this protein can be ubiquitinated by the E2 ligase UBE2N and the E3 ligases RNF8 and RNF168 as result of double-stranded DNA breaks to recruit repair proteins a damaged site. H2AX is phosphorylated soon after the appearance of double-stranded breaks. Hyperphoshorylation of H2AX may be linked to chromatin fragmentation prior to apoptosis.

Especificidad

This antibody recognizes Histone H2A.X when ubiquitylated at Lys119.

Inmunógeno

Epitope: Lys119

Linear peptide containing ubiquitylated Lys119 of human Histone H2A.X.

Aplicación

Anti-Ubiquityl Histone H2A.X (Lys119) Antibody is a rabbit polyclonal antibody for detection of Ubiquityl Histone H2A.X (Lys119) also known as H2AXK119Ub, Histone H2A.X Ubiquityl K119) & has been validated in WB, IP, ICC.

Immunocytochemsitry Analysis: 1:500 dilution from a representative lot detected Histone H2A.X in RAW264.7 and NIH/3T3 cells.

Immunoprecipitation Analysis: 5 µl from a representative lot immunoprecipitated Histone H2A.X in HeLa acid extract.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Calidad

Evaluated by Western Blot in HeLa acid extract.

Western Blot Analysis: 1:1,000 dilution of this antibody detected Histone H2A.X on 10 µg of HeLa acid extract.

Descripción de destino

~ 23 kDa

Forma física

Unpurified

Unpurified rabbit polyclonal containing serum with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at -20°C from date of receipt.

Nota de análisis

Control
HeLa acid extract

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1