69671

Thrombin, Restriction Grade

• Sinónimos: Thrombin, Restriction Grade
• Número de CAS: 9002-04-4
• Número MDL: MFCD18251359
• Código UNSPSC: 12352202
• NACRES: NA.54

Propiedades

• origen biológico: human plasma
• Nivel de calidad: 400
• Formulario: liquid
• fabricante / nombre comercial: Novagen^®
• condiciones de almacenamiento: OK to freeze; avoid repeated freeze/thaw cycles
• técnicas: protein extraction: suitable
• idoneidad: suitable for additive or modifier in the separation of proteins or peptides
• aplicaciones: life science and biopharma
• Condiciones de envío: wet ice
• temp. de almacenamiento: −20°C

Descripción

Descripción general

Restriction Grade Thrombin is a highly purified preparation qualified to specifically cleave target proteins produced with appropriate vectors. This preparation is functionally tested for activity with fusion proteins and is free of detectable contaminating proteases. Prepared from human plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV. Thrombin is supplied as a solution in 50% glycerol and includes 10X Thrombin Cleavage Buffer and a Cleavage Control Protein.

Componentes

•50 UThrombin

•1 ml10X Thrombin Cleavage Buffer

•2 ml1X Thrombin Dilution/Storage Buffer

•10 µgCleavage Control Protein

Advertencia

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Definición de unidad

One unit is defined as the amount of enzyme needed to cleave 1 mg of fusion protein in 16 hours at 20°C in a 200 µl reaction containing 20 mM Tris-HCl pH 8.4, 150 mM NaCl, 2.5 mM CaCl₂, 50 µg fusion protein.

Nota de preparación

Prepared from human plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Información legal

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1