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Merck

RECOMT

Sigma-Aldrich

Thrombin CleanCleave Kit

Sinónimos:

Thrombin Cleavage Kit

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About This Item

Código UNSPSC:
12352200
NACRES:
NA.56

Formulario

suspension

Nivel de calidad

mol peso

37 kDa

técnicas

protein purification: suitable

Condiciones de envío

wet ice

temp. de almacenamiento

2-8°C

Descripción general

The Thrombin CleanCleave Kit contains a 50% suspension fo thrombin-agarose produced by immobilizing bovine thrombin and is designed for cleavage of recombinant fusion proteins

Aplicación

For cleaving a tag from a recombinant fusion protein which contains the thrombin recognition sequence. Thrombin-agarose is compatible with recombinant proteins expressed in various types of expression systems.

Características y beneficios

  • Fast, efficient cleavage in as little as 2 hours
  • Thrombin is covalently bound to agarose for easy removal.
  • The robust cleavage reaction is effective at temperatures from 4 °C to 37 °C and over a wide range of pH and ionic strengths.
  • Cleave tags even in the presence of 0.1% Triton, 1 M urea or 5 mM EDTA
  • Thrombin-agarose is reusable.

Cantidad

200 μl of a 50% slurry of thrombin-agarose cleaves >85% of 1 mg of fusion protein.

Información legal

CleanCleave is a trademark of Sigma-Aldrich Co. LLC
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Solo componentes del kit

Referencia del producto
Descripción

  • Thrombin Cleavage Buffer, 10× 10 mL

Producto relacionado

Referencia del producto
Descripción
Precios

Código de clase de almacenamiento

10 - Combustible liquids

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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