RECOMT
Thrombin CleanCleave™ Kit
Sinónimos:
Thrombin Cleavage Kit
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About This Item
Código UNSPSC:
12352200
NACRES:
NA.56
Productos recomendados
Formulario
suspension
mol peso
37 kDa
técnicas
protein purification: suitable
Condiciones de envío
wet ice
temp. de almacenamiento
2-8°C
Descripción general
The Thrombin CleanCleave Kit contains a 50% suspension fo thrombin-agarose produced by immobilizing bovine thrombin and is designed for cleavage of recombinant fusion proteins
Aplicación
For cleaving a tag from a recombinant fusion protein which contains the thrombin recognition sequence. Thrombin-agarose is compatible with recombinant proteins expressed in various types of expression systems.
Características y beneficios
- Fast, efficient cleavage in as little as 2 hours
- Thrombin is covalently bound to agarose for easy removal.
- The robust cleavage reaction is effective at temperatures from 4 °C to 37 °C and over a wide range of pH and ionic strengths.
- Cleave tags even in the presence of 0.1% Triton™, 1 M urea or 5 mM EDTA
- Thrombin-agarose is reusable.
Cantidad
200 μl of a 50% slurry of thrombin-agarose cleaves >85% of 1 mg of fusion protein.
Información legal
CleanCleave is a trademark of Sigma-Aldrich Co. LLC
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
Solo componentes del kit
Referencia del producto
Descripción
- Thrombin Cleavage Buffer, 10× 10 mL
Producto relacionado
Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
10 - Combustible liquids
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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