676604

PhosphoDetect Anti-VASP (pSer¹⁵⁷) Mouse mAb (5C6)

lyophilized, clone 5C6, Calbiochem^®

• Sinónimos: Anti-Vasodilator-stimulated Phosphoprotein, Activated
• Código UNSPSC: 12352203

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: 5C6, monoclonal
• formulario: lyophilized
• contiene: ≤0.1% sodium azide as preservative
• reactividad de especies: human, mouse
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze
• isotipo: IgG1
• Condiciones de envío: ambient
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: phosphorylation (pSer157)
• Información sobre el gen: human ... VASP(7408)

Descripción

Descripción general

Mouse monoclonal antibody purified from serum-free cell culture supernatant by thiophilic adsorption and size exclusion chromatography. Recognizes the the ~50 kDa VASP protein phosphorylated at Ser¹⁵⁷.

Recognizes the the ~50 kDa VASP protein phosphorylated at Ser¹⁵⁷.

This PhosphoDetect Anti-VASP (pSer¹⁵⁷) Mouse mAb (5C6) is validated for use in ELISA, Immunoblotting, ICC, IP for the detection of VASP (pSer¹⁵⁷).

Inmunógeno

Human

a synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹⁵⁷ phosphorylation site of human VASP

Aplicación

ELISA (0.05 µg/ml)

Immunoblotting (1 µg/ml, colorimetric; 0.1 µg/ml, chemiluminescence)

Immunocytochemistry (1-10 µg/ml)

Immunoprecipitation (1-10 µg/10⁶ vanadate-treated A431 cells)

Advertencia

Toxicity: Standard Handling (A)

Forma física

Lyophilized from PBS, PEG, sucrose.

Reconstitución

Reconstitute in 1 ml sterile, dH₂O, 15 min, room temperature. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solutions.

Nota de análisis

Positive Control
Vanadate-treated A431 cells

Otras notas

Bear, J.E., et al. 2001. Curr. Opin. Cell. Biol.13, 158.
Lawrence, D.W., and Pryzwansky, K.B. 2001. J. Immunol.166, 5550.
Burkhardt, M., et al. 2000. J. Biol. Chem.275, 33536.
Harbeck, B., et al. 2000. J. Biol. Chem.275, 40.
Smolenski, A., et al. 2000. J. Biol. Chem.275, 25723.

Does not cross-react with non-phosphorylated VASP or with unrelated phosphorylation sites. For immunocytochemistry, fixation protocol may include up to 0.5% formaldehyde. Variables associated with assay conditions will dictate the proper working dilution.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable