525295
PhosphoDetect Anti-Phosphotyrosine Mouse mAb (PY20)
liquid, clone PY20, Calbiochem®
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About This Item
Código UNSPSC:
12352203
Productos recomendados
origen biológico
mouse
Nivel de calidad
forma del anticuerpo
purified antibody
tipo de anticuerpo
primary antibodies
clon
PY20, monoclonal
formulario
liquid
contiene
≤0.1% sodium azide as preservative
reactividad de especies (predicha por homología)
all
fabricante / nombre comercial
Calbiochem®
condiciones de almacenamiento
OK to freeze
avoid repeated freeze/thaw cycles
isotipo
IgG2b
Condiciones de envío
wet ice
temp. de almacenamiento
−20°C
modificación del objetivo postraduccional
phosphorylation (pTyr)
Información sobre el gen
human ... PID1(55022)
Descripción general
Purified mouse monoclonal antibody. Recognizes a broad spectrum of tyrosine-phosphorylated proteins in their native and denatured states.
Recognizes a broad spectrum of tyrosine-phosphorylated proteins in their native and denatured states in crude extracts.
This PhosphoDetect Anti-Phosphotyrosine Mouse mAb (PY20) is validated for use in ELISA, Immunoblotting, Frozen Sections, ICC, IP for the detection of Phosphotyrosine.
Inmunógeno
KLH conjugated to phosphotyrosine, glycine, and alanine
Aplicación
ELISA (see comments)
Immunoblotting (1:500-1:1000, see application references)
Frozen Sections (see application references)
Immunocytochemistry (see application references)
Immunoprecipitation (see comments)
Immunoblotting (1:500-1:1000, see application references)
Frozen Sections (see application references)
Immunocytochemistry (see application references)
Immunoprecipitation (see comments)
Envase
Please refer to vial label for lot-specific concentration.
Advertencia
Toxicity: Standard Handling (A)
Forma física
In 10 mM sodium phosphate, pH 7.4.
Reconstitución
Following initial thaw, aliquot and freeze (-20°C).
Nota de análisis
Positive Control
Any cell lysate or tissue extract
Any cell lysate or tissue extract
Otras notas
Binding is inhibited by phosphotyrosine. Does not react with threonine or serine phosphorylated residues on proteins. Do not use milk as a blocking agent with this antibody. Milk contains many phosphotyrosines and will adsorb the antibody. This antibody has also been reported to work for ELISA and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
Glenney, J.R., et al. 1988. J. Immunol. Methods109, 277.
Información legal
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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