05-591

Anti-Akt/PKB Antibody, PH Domain, clone SKB1

clone SKB1, Upstate^®, from mouse

• Sinónimos: Anti-AKT, Anti-PKB, Anti-PKB-ALPHA, Anti-PRKBA, Anti-RAC, Anti-RAC-ALPHA
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: affinity purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: SKB1, monoclonal
• reactividad de especies: rat, human, mouse
• envase: antibody small pack of 25 μg
• fabricante / nombre comercial: Upstate^®
• técnicas: flow cytometry: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotipo: IgG
• Nº de acceso NCBI: NM_005163
• Nº de acceso UniProt: P31749
• Condiciones de envío: dry ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: mouse ... Akt1(11651)

Descripción

Descripción general

Akt (protein kinase B), a serine/threonine kinase, has emerged as a critical enzyme in signal transduction pathways involved in cell proliferation, apoptosis, angiogenesis, and diabetes. In mammals three isoforms of Akt (α, β, γ or Akt 1, 2, 3) are reported that exhibit a high degree of homology, but differ slightly in the localization of their regulatory phosphorylation sites. Akt α is the predominant isoform in most tissues, whereas the highest expression of Akt β is observed in the insulin-responsive tissues, and Akt γ is abundant in brain tissue. Each Akt isoform is composed of three functionally distinct regions: an N-terminal pleckstrin homology (PH) domain that provides a lipid-binding module to direct Akt to PIP2 and PIP3, a central catalytic domain, and a C-terminal hydrophobic motif.

Aplicación

Detect Akt/PKB using this Anti-Akt/PKB Antibody, PH Domain, clone SKB1 validated for use in FC, IC, IP & WB.

Calidad

routinely evaluated by immunoblot on RIPA lysates from A431 cells

Descripción de destino

60 kDa

Forma física

Format: Purified

Protein G Purified immunoglobulin in Immunoaffinity Purified immunoglobulin in 0.02 M phosphate buffer, pH 7.6, 0.25M NaCl, 0.1% sodium azide and 30% glycerol.

Nota de análisis

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany