517310
Anti-P-Glycoprotein Mouse mAb (C219)
liquid, clone C219, Calbiochem®
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About This Item
Código UNSPSC:
12352203
Productos recomendados
origen biológico
mouse
Nivel de calidad
forma del anticuerpo
purified antibody
tipo de anticuerpo
primary antibodies
clon
C219, monoclonal
formulario
liquid
contiene
≤0.1% sodium azide as preservative
reactividad de especies
rat, hamster, human, mouse
fabricante / nombre comercial
Calbiochem®
condiciones de almacenamiento
OK to freeze
avoid repeated freeze/thaw cycles
isotipo
IgG1
Condiciones de envío
wet ice
temp. de almacenamiento
−20°C
Información sobre el gen
human ... ABCB1(5243)
Descripción general
Purified mouse monoclonal antibody. Recognizes the ~170 kDa MDR1 and MDR3 isoforms of P-glycoprotein.
Recognizes the ~170 kDa MDR1 and MDR3 isoforms of P-glycoprotein.
This Anti-P-Glycoprotein Mouse mAb (C219) is validated for use in FC, Frozen Sections, Immunoblotting, ICC, IP, Paraffin Sections for the detection of P-Glycoprotein.
Inmunógeno
Hamster and Human
SDS-solubilized plasma membranes of a multidrug resistant (MDR) Chinese hamster ovary (CHO) cell line and a multidrug resistant human cell line
Aplicación
Flow Cytometry (5-10 µg/1 x 10⁶ cells)
Frozen Sections (5-10 µg/ml)
Immunoblotting (1-10 µg/ml, see application references, see comments)
Immunocytochemistry (see comments)
Immunoprecipitation (see comments)
Paraffin Sections (1-10 µg/ml)
Frozen Sections (5-10 µg/ml)
Immunoblotting (1-10 µg/ml, see application references, see comments)
Immunocytochemistry (see comments)
Immunoprecipitation (see comments)
Paraffin Sections (1-10 µg/ml)
Envase
Please refer to vial label for lot-specific concentration.
Advertencia
Toxicity: Standard Handling (A)
Forma física
In PBS, 1% BSA.
Reconstitución
Following initial thaw, aliquot and freeze (-20°C).
Nota de análisis
Positive Control
Cell lines or frozen tissue, such as liver or colon
Cell lines or frozen tissue, such as liver or colon
Otras notas
Also recognizes ~150 kDa sister P-glycoprotein (SPGP). Frozen sections should be fixed in acetone for 10 min at -20°C. Formalin-fixed, paraffin tissue sections should be deparaffinized and rehydrated by conventional means. Boiling samples may reduce visibility of the protein by immunoblotting (see application references). This antibody is also reported to work for immunocytochemistry and immunoprecipitation. A ~200 kDa protein that migrates in the same position as myosin is also seen by immunoblotting. Variables associated with assay conditions will dictate the proper working dilutions.
Beck, W.T., et al. 1996. Cancer Res. 56, 3010.
Toth, K., et al. 1992. Am. J. Pathol. 140, 1009.
Schinkel, A.H., et al. 1991. Cancer Res. 51, 2628.
Chan, H.S.L., et al. 1988. Lab Invest. 59, 870.
Toth, K., et al. 1992. Am. J. Pathol. 140, 1009.
Schinkel, A.H., et al. 1991. Cancer Res. 51, 2628.
Chan, H.S.L., et al. 1988. Lab Invest. 59, 870.
Información legal
ImmunoblottingLee, G., et al. 2001. J. Pharmacol. Exp. Ther.299, 204.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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