484400

Nitrocefin

≥95% (UV), solid, β-lactamase substrate, Calbiochem^®

• Sinónimos: Nitrocefin, 3-(2,4-Dinitrostyryl)-(6R, 7R)-7-(2-thienylacetamido)-ceph-3-em-4-carboxylic Acid, E-isomer
• Fórmula empírica (notación de Hill): C₂₁H₁₆N₄O₈S₂
• Número de CAS: 41906-86-9
• Peso molecular: 516.50
• Código UNSPSC: 12352200
• NACRES: NA.21

Propiedades

• product name: Nitrocefin, A chromogenic β-lactamase substrate that undergoes distinctive color change from yellow as the amide bond in the β-lactam ring is hydrolyzed by β-lactamase.
• Nivel de calidad: 200
• Análisis: ≥95% (UV)
• formulario: solid
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze; protect from light
• color: orange-yellow
• Condiciones de envío: ambient
• temp. de almacenamiento: −20°C
• InChI: 1S/C21H16N4O8S2/c26-16(9-14-2-1-7-34-14)22-17-19(27)23-18(21(28)29)12(10-35-20(17)23)4-3-11-5-6-13(24(30)31)8-15(11)25(32)33/h1-8,17,20H,9-10H2,(H,22,26)(H,28,29)/b4-3+/t17-,20-/m1/s1
• Clave InChI: LHNIIDJCEODSHA-OQRUQETBSA-N

Descripción

Descripción general

A chromogenic β-lactamase substrate that undergoes distinctive color change from yellow (λ_max = 390 nm at pH 7.0) to red (λ_max = 486 nm at pH 7.0) as the amide bond in the β-lactam ring is hydrolyzed by β-lactamase. Nitrocefin is sensitive to hydrolysis by all known lactamases produced by Gram-positive and Gram-negative bacteria. Also useful for the detection of β-lactamase patterns from bacterial cell extracts by isoelectric focusing. Has been used in competitive inhibition studies in developmental work on β-lactamase-resistant antibiotics.

Envase

Packaged under inert gas

Advertencia

Toxicity: Flammable (J)

Nota de preparación

Preparing a Nitrocefin (500 µg/ml) Solution

• Dissolve 1 mg Nitrocefin in 100 µl dimethylsulfoxide (DMSO) and vortex.

• Add 1.9 ml phosphate buffer (100 mM, pH 7) to produce 2 ml total volume.

• This yields a working Nitrocefin solution of 500 µg/ml (~1 mM), which is suitable for most applications.

• Nitrocefin, particularly in solution, is very sensitive to light.

Reconstitución

Following reconstitution, aliquot and freeze (-20°C). Stock solution may be stored at -20°C for up to 2 weeks.

Otras notas

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Guay, R., et al. 1980. IRCS Med. Science 8, 209.
King, A., et al. 1980. Antimicrob. Agents Chemother. 17, 165.
Matthew, M., et al. 1975. J. Gen. Microbiol. 88, 169.
O’Callaghan, C.H., et al. 1972. Antimicrob. Agents Chemother. 1, 283.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Pictogramas: GHS05,GHS07
• Palabra de señalización: Danger
• Frases de peligro: H315,H317,H318
• Consejos de prudencia: P261 - P264 - P272 - P280 - P302 + P352 - P305 + P351 + P338
• Clasificaciones de peligro: Eye Dam. 1 - Skin Irrit. 2 - Skin Sens. 1
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3