475911

Myeloperoxidase, Human Polymorphonuclear Leukocytes

A lysosomal enzyme that catalyzes oxidations by hydrogen peroxide, including MPO-chloride-mediated killing of microbes and tumor cells, inactivation of chemotactic factors, and cross-linking of proteins.

• Sinónimos: Myeloperoxidase, Human Polymorphonuclear Leukocytes, MPO
• Número de CAS: 9003-99-0
• Comisión internacional de enzimas: 1.11.1.7 (BRENDA, IUBMB)
• Código UNSPSC: 12352202
• NACRES: NA.25

Propiedades

• origen biológico: human
• Nivel de calidad: 200
• Análisis: ≥95% (SDS-PAGE)
• formulario: lyophilized
• actividad específica: 150-200 units/mg protein
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze
• solubilidad: water: soluble (volume is lot-specific and can be found on the product label)
• Condiciones de envío: wet ice
• temp. de almacenamiento: −20°C
• InChI: 1S/H2O3/c1-3-2/h1-2H
• Clave InChI: JSPLKZUTYZBBKA-UHFFFAOYSA-N

Descripción

Descripción general

Native, human myeloperoxidase from polymorphonuclear leukocytes. A lysosomal enzyme that catalyzes oxidations by hydrogen peroxide, including MPO-chloride-mediated killing of microbes and tumor cells, inactivation of chemotactic factors, and cross-linking of proteins. MPO catalyzes the formation of hypochlorous acid that kills bacteria either by binding the halide ion to intracellular constituents or by oxidation of bacterial lipids and proteins.

Advertencia

Toxicity: Standard Handling (A)

Definición de unidad

One unit is defined as the amount of enzyme that will reduce 1.0 µmol H₂O₂ per min at 25°C, pH 6.0.

Forma física

Lyophilized from 100-700 mM NaCl, 50 mM sodium acetate buffer, pH 6.0.

Nota de preparación

Further dilute in 100 mM NaCl, 50 mM sodium acetate, pH 6.0. Enzyme may precipitate out of solution in low ionic strength buffers or in distilled H₂O.

Reconstitución

Following reconstitution, aliquot and freeze (-20°C or -70°C). Stock solutions are stable for up to 1 year at -20°C or -70°C.

Otras notas

Nauseef, W.M., et al. 1998. J. Leukocyte Biol. 63, 264.
Panasenko, O.M., et al. 1994. Free Radic. Biol. Med. 16, 143.
Mulligan, M.S., et al. 1992. Br. J. Pharmacol.107, 1159.
Sharonov, B.P., and Churilova, I.V. 1992. Biochem. Biophys. Res. Commun.189, 1129.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Pictogramas: GHS08
• Palabra de señalización: Danger
• Frases de peligro: H334
• Consejos de prudencia: P261 - P284 - P501
• Clasificaciones de peligro: Resp. Sens. 1
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1