444213

MMP-2, Proenzyme, Human Rheumatoid Synovial Fibroblast

• Sinónimos: Matrix Metalloproteinase 2, Gelatinase A, 72 kDa Type IV Collagenase
• Comisión internacional de enzimas: 3.4.24.24 (BRENDA, IUBMB)
• Código UNSPSC: 12352202
• NACRES: NA.77

Propiedades

• Ensayo: ≥90% (SDS-PAGE)
• Nivel de calidad: 100
• Formulario: liquid
• actividad específica: ≥600 mU/mg protein
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze; avoid repeated freeze/thaw cycles
• solubilidad: aqueous buffer: soluble
• actividad extraña: other MMP activity, none detected
• Condiciones de envío: wet ice
• temp. de almacenamiento: −70°C

Descripción

Descripción general

Native pro-MMP-2 from cultured, human rheumatoid synovial fibroblasts. Highly purified native enzyme derived from cell culture. Secreted by several cell types. Exists in a stable, but non-covalent 1:1 complex with TIMP-2. Requires activation prior to use.

Native pro-MMP-2 from cultured, human rheumatoid synovial fibroblasts. Secreted by several cell types. Requires activation just prior to use.

Note: 1 mU = 1 milliunit.

Envase

Please refer to vial label for lot-specific concentration.

Advertencia

Toxicity: Standard Handling (A)

Definición de unidad

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol 2,4-DNP-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH per min at 37°C, pH 7.0.

Forma física

In 200 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, 1 µM ZnCl₂, 0.05% Brij^® 35 Detergent, 0.05% NaN₃, pH 7.0.

Nota de preparación

Prepared from culture medium of human rheumatoid synovial fibroblasts that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitución

Following initial thaw, aliquot and freeze (-70°C).

Otras notas

Buisson-Leqendre, N., et al. 2004. Arth. Rheumat.50, 2151.
Morgunova, E., et al. 2002. Proc. Natl. Acad. Sci. USA99, 7414.
Kolkenbrock, H., et al. 1997. Biol. Chem. 378, 71.

Información legal

Brij is a registered trademark of Croda International PLC

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable