407617

Interleukin-1β, Mouse, Recombinant, E. coli

• Código UNSPSC: 12352202
• NACRES: NA.41

Propiedades

• descripción: Merck USA index - 14, 4993
• Nivel de calidad: 100
• Ensayo: ≥97% (SDS-PAGE)
• Formulario: lyophilized
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze
• impurezas: ≤1.0 EU/μg Endotoxin (EU/μg IL-1β)
• Condiciones de envío: ambient
• temp. de almacenamiento: −70°C

Descripción

Descripción general

Recombinant, mouse interleukin-1β expressed in E. coli. Suppresses apoptosis in rat ovarian follicles. Involved in inflammatory and immune responses and promotes wound healing. Interleukin-1 is a protein produced by stimulated macrophages and many other cell types. The following biological activities are attributed to IL-1: activation of T cells resulting in the production of IL-2 and expression of IL-2 receptors; initiation of Acute Phase Response by induction of hepatic protein synthesis; increased proteolysis of muscle proteins, amino acid mobilization, and caloric and oxygen utilization; induction of bone resorption; stimulation of fibroblast proliferation; induction of slow-wave sleep; fever production; induction of prostaglandin and leukotriene production; stimulation of thymocyte proliferation; induction of B cell proliferation and maturation and increased immunoglobulin synthesis; induction of collagenase and other proteinases in synovial fibroblasts and articular chrondrocytes, suggesting a role in pathological states such as arthritis; activation of neutrophils. 1 µg is equivalent to 1.1 x 10⁶ WHO units.

Acciones bioquímicas o fisiológicas

ED₅₀= 5-10 pg/ml as measured in a cell proliferation assay

Advertencia

Toxicity: Standard Handling (A)

Forma física

Lyophilized from sterile-filtered PBS, 50 µg BSA/µg IL-1β.

Reconstitución

Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C.

Reconstitute to a concentration of ≥10 µg/ml with sterile PBS containing ≥0.1% HSA or BSA.

Otras notas

Chun, S.Y., et al. 1995. Endocrinology136, 3120.
Weidmann, B., et al. 1994. Nature370, 434.
Dinarello, C.A., and Wolff, S.M. 1993. N. Engl. J. Med.328, 106.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable