371955-M

H-7, Dihydrochloride - CAS 108930-17-2 - Calbiochem

A broad-based, cell-permeable, reversible, ATP-competitive serine/threonine kinase inhibitor.

• Sinónimos: H-7, Dihydrochloride - CAS 108930-17-2 - Calbiochem, 1-(5-Isoquinolinesulfonyl)-2-methylpiperazine, 2HCl
• Fórmula empírica (notación de Hill): C₁₄H₁₇N₃O₂S · 2HCl
• Número de CAS: 108930-17-2
• Peso molecular: 364.29
• Número MDL: MFCD00036961
• Código UNSPSC: 12352200

Propiedades

• Nivel de calidad: 100
• Análisis: ≥98% (TLC)
• formulario: lyophilized solid
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze; protect from light
• color: light yellow
• solubilidad: water: 20 mg/mL
• temp. de almacenamiento: 2-8°C
• InChI: 1S/C14H17N3O2S.2ClH/c1-11-9-16-7-8-17(11)20(18,19)14-4-2-3-12-10-15-6-5-13(12)14;;/h2-6,10-11,16H,7-9H2,1H3;2*1H
• Clave InChI: OARGPFMFRLLKPF-UHFFFAOYSA-N

Descripción

Descripción general

A broad-based, cell-permeable, reversible, ATP-competitive serine-threonine kinase inhibitor. Potent inhibitor of myosin light chain kinase (K_i = 97 µM), protein kinase A (K_i = 3.0 µM), protein kinase C (K_i = 6 µM), and protein kinase G (K_i = 5.8 µM). Induces apoptotic DNA fragmentation and cell death in HL-60 human promyelocytic leukemia cells. Also inhibits telomerase activity in quercetin-, H-89-, or herbimycin A-treated NPC-076 cells.

A broad-based, cell-permeable, reversible, ATP-competitive serine/threonine kinase inhibitor. Inhibits protein kinase A (K_i = 3.0 µM), myosin light chain kinase (K_i = 97 µM), protein kinase C (K_i = 6.0 µM), and protein kinase G (K_i = 5.8 µM). Induces apoptotic DNA fragmentation and cell death in HL-60 human promyelocytic leukemia cells. Also inhibits telomerase activity in quercetin-, H-89-, or herbimycin A-treated NPC-076 cells.

Acciones bioquímicas o fisiológicas

Primary Target
MLCK

Target K_i: 3.0 µM, 97 µM, 6.0 µM, 5.8 µM against protein kinase A , myosin light chain kinase, protein kinase C , and protein kinase G, respectively

Advertencia

Toxicity: Standard Handling (A)

Nota de preparación

Further dilute with aqueous buffers just prior to use.

Reconstitución

Following reconstitution, store in the refrigerator (4°C). Aqueous stock solutions are stable for up to 6 months at 4°C.

Otras notas

Ku, W.-C., et al. 1997. Biochem. Biophys. Res. Commun. 241, 730.
Jarvis, W.D., et al. 1994. Cancer Res.54, 1707.
Bergstrand, H., et al. 1992. J. Pharmacol. Exp. Therap.263, 1334.
Gimond, C., et al. 1992. Exp. Cell Res.203, 365.
Quick, J., et al. 1992. Biochem. Biophys. Res. Commun.187, 657.
Bouli, N.M., and Davis, M. 1990. Brain Res.525, 198.
Takahashi, I., et al. 1990. J. Pharmacol. Exp. Ther.255, 1218.
Schachtele, C., et al. 1988. Biochem. Biophys.Res. Commun.151, 542.
Kawamoto, S., and Hidaka, H. 1984. Biochem. Biophys. Res. Commun.125, 258.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable