324715

Endoproteinase Lys-C, Sequencing Grade, Lysobacter enzymogenes

• Sinónimos: Endoproteinase Lys-C, Sequencing Grade, Lysobacter enzymogenes
• Número de CAS: 72561-05-8
• Comisión internacional de enzimas: 3.4.21.50 (BRENDA, IUBMB)
• Número MDL: MFCD02253020
• Código UNSPSC: 12352202

Propiedades

• grado: Proteomics Grade
• Nivel de calidad: 100
• Ensayo: ≥90% (SDS-PAGE)
• Formulario: lyophilized
• actividad específica: ≥200 units/mg protein
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze; desiccated (hygroscopic)
• Condiciones de envío: ambient
• temp. de almacenamiento: 2-8°C

Descripción

Descripción general

Native endoproteinase Lys-C from Lysobacter enzymogenes. Serine protease that specifically hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine in peptides and proteins. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Inhibited by aprotinin, DFP, leupeptin, and TLCK.

Native endoproteinase Lys-C from Lysobacter enzymogenes. Serine protease that specifically hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine in peptides and proteins. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.

Advertencia

Toxicity: Harmful (C)

Definición de unidad

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Tos-Gly-Pro-Lys-pNA per min at 25°C, pH 7.7.

Forma física

Lyophilized from 50 mM HEPES, 10 mM EDTA, 5 mg/ml raffinose, pH 8.0.

Reconstitución

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.

Reconstitute in 50 µl of distilled H₂O.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany