300260

Diphenyleneiodonium Chloride

≥97% (HPLC), crystalline solid, eNOS inhibitor, Calbiochem^®

• Sinónimos: Diphenyleneiodonium Chloride, DPI
• Fórmula empírica (notación de Hill): C₁₂H₈ClI
• Número de CAS: 4673-26-1
• Peso molecular: 314.55
• Número MDL: MFCD00214165
• Código UNSPSC: 12352200
• NACRES: NA.77

Propiedades

• Nombre del producto: Diphenyleneiodonium Chloride, A cell-permeable, irreversible inhibitor of endothelial nitric oxide synthase (eNOS).
• Nivel de calidad: 100
• Ensayo: ≥97% (HPLC)
• Formulario: crystalline solid
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze; protect from light
• color: off-white
• solubilidad: DMSO: 5 mg/mL
• Condiciones de envío: ambient
• temp. de almacenamiento: −20°C
• InChI: 1S/C12H8I.ClH/c1-3-7-11-9(5-1)10-6-2-4-8-12(10)13-11;/h1-8H;1H/q+1;/p-1
• Clave InChI: FCFZKAVCDNTYID-UHFFFAOYSA-M

Descripción

Descripción general

A cell-permeable, irreversible endothelial nitric oxide synthase inhibitor. Inhibits iNOS in macrophages (IC₅₀ = 5 nM). Shown to inhibit acetylcholine-induced relaxation of preconstricted rat thoracic aortic rings (IC₅₀ = 180 nM). Blocks K⁺ and Ca²⁺ currents in arterial smooth muscle cells. Inhibitor of mitochondrial NADH-ubiquinone oxidoreductase. Inhibits other NADH utilizing flavoproteins, such as NADPH oxidase in human neutrophils and macrophages. Causes pressor and tachycardiac effects via indirect activation of the sympathetic nervous system.

A cell-permeable, irreversible inhibitor of endothelial nitric oxide synthase (eNOS). Shown to inhibit acetylcholine-induced relaxation of preconstricted rat thoracic aortic rings (IC₅₀ = 180 nM). Also inhibits iNOS in macrophages (IC₅₀ = 50 nM). Blocks K⁺ and Ca²⁺ currents in arterial smooth muscle cells. Inhibitor of mitochondrial NADPH-ubiquinone oxido-reductase.

Acciones bioquímicas o fisiológicas

Cell permeable: yes

Primary Target
eNOS

Product does not compete with ATP.

Reversible: no

Target IC₅₀: 180 nM against endothelial nitric oxide synthase (eNOS)

Advertencia

Toxicity: Carcinogenic / Teratogenic (D)

Nota de preparación

Slight warming is required for complete solubility.

Reconstitución

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Otras notas

Wang, Y.X., et al. 1995. Br. J. Pharmacol.114, 194.
Majander, A., et al. 1994. J. Biol. Chem. 269, 21037.
Weir, E.K., et al. 1994. J. Appl. Physiol. 76, 2611.
Wang, Y.X., et al. 1993. Br. J. Pharmacol.110, 1232.
Wang, Y.X., and Pang, C.C. 1993. Br. J. Pharmacol.109, 1186.
Stuehr, D.J., et al. 1991. FASEB J.5, 98.
Handcock, J.T., and Jones, O.T.G. 1987. Biochem. J.242, 103.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable