268298

DAPI, Dihydrochloride

Cell-permeable DNA-binding dye.

• Sinónimos: DAPI, Dihydrochloride, 4ʹ,6-Diamidino-2-phenylindole, 2HCl
• Fórmula empírica (notación de Hill): C₁₆H₁₅N₅ · 2HCl
• Número de CAS: 28718-90-3
• Peso molecular: 350.25
• Número MDL: MFCD00012681
• Código UNSPSC: 12352200
• NACRES: NA.54

Propiedades

• Nivel de calidad: 100
• descripción: RTECS - NL5995050
• Ensayo: ≥90% (HPLC)
• Formulario: solid
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze; desiccated (hygroscopic); protect from light
• color: yellow
• solubilidad: water: 5 mg/mL; ethanol: soluble; methanol: soluble
• fluorescencia: λ_ex ~359 nm; λ_em ~461 nm
• Condiciones de envío: ambient
• temp. de almacenamiento: −20°C
• cadena SMILES: [Cl-].[Cl-].[nH]1c2c(cc1c3ccc(cc3)C(=N)N)ccc(c2)C(=N)N.[H+].[H+]
• InChI: 1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
• Clave InChI: FPNZBYLXNYPRLR-UHFFFAOYSA-N

Descripción

Descripción general

Cell-permeable DNA-binding dye. Binds preferentially to DNA rich in adenine and thymine. Used in flow cytometry for measuring nuclear DNA content or for sorting isolated chromosomes. Useful for microscopic detection of nuclei and nuclear DNA in normal and apoptotic cells. Can also be used to detect mycoplasma. A reversible inhibitor of S-adenosyl-L-methionine decarboxylase and diamine oxidase. Also available as a 5 mg/ml mM solution in H₂O (Cat. No. 508741).

Fluorescent dye specific for DNA. Binds preferentially to DNA rich in adenine and thymidine (excitation max. = 359 nm, emission max. = 461 nm). Useful for microscopic analysis of nuclei and nuclear DNA in apoptotic cells. Can also be used to detect mycoplasma. Reversible inhibitor of S-adenosyl-L-methionine decarboxylase and diamine oxidase. Cells are typically stained at 1 µg/ml.

Acciones bioquímicas o fisiológicas

Cell permeable: no

Primary Target
Binds preferentially to DNA rich in adenine and thymine

Product does not compete with ATP.

Reversible: yes

Advertencia

Toxicity: Standard Handling (A)

Reconstitución

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 6 weeks at 4°C or for up to 6 months at -20°C.

Otras notas

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Pictogramas: GHS07
• Palabra de señalización: Warning
• Frases de peligro: H315,H317,H335
• Consejos de prudencia: P261 - P264 - P271 - P272 - P280 - P302 + P352
• Clasificaciones de peligro: Skin Irrit. 2 - Skin Sens. 1A - STOT SE 3
• Órganos de actuación: Respiratory system
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable