Saltar al contenido
Merck
Todas las fotos(2)

Documentos clave

23-028-M

Sigma-Aldrich

IkBa Protein, activated, 10 µg

Activated, full length, recombinant human IκBα. N-terminal c-Myc, 6His-tagged. Phosphorylated in vitro using IKKα, active. For use in Enzyme Assays. Behaves as a substrate in ubiquitin assays.

Sinónimos:

NF-kappa-B inhibitor alpha, I-kappa-B-alpha, Major histocompatibility complex enhancer-binding protein MAD3

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización


About This Item

Código UNSPSC:
12352202
eCl@ss:
32160405
NACRES:
NA.41

origen biológico

human

Nivel de calidad

100
300

recombinante

expressed in E. coli

fabricante / nombre comercial

Upstate®

técnicas

activity assay: suitable

Nº de acceso UniProt

Descripción general

IκBα is a cellular protein that inhibits the transcription factor NF-κB. NF-κB plays a central role in cellular stress and inflammatory responses by controlling cytokine-inducible gene expression and lymphocyte stimulation by antigens. In non-stimulated cells NF-κB is sequestered in the cytoplasm via the inhibitory protein IκBα, which obscures the nuclear localization signal of NF-κB and also blocks its ability to bind DNA. In response to various stimuli, including viral infection, ultraviolet radiation and inflammatory cytokines, IκBα is phosphorylated by the protein kinase complex IKK, which triggers its rapid degradation by ubiquitin mediated proteolysis. The liberated NF-κB then translocates to the nucleus and activates the expression of target genes. Mutations in the gene for IκBα result in the functional impairment of NF-κB which causes a decreased production of proinflammatory cytokines and certain interferons, rendering patients susceptible to infection.
N-terminal GST-tagged, recombinant human Smurf1 full length, expressed in E coli.

Aplicación

Ubiquitination Cascade Comoponent: Substrate

Forma física

Purified using immobilized metal affinity chromatography.

Otras notas

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Información legal

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

nwg

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico