382061

Bisbenzimide H 33258 Fluorochrome, Trihydrochloride

Membrane-permeable, adenine-thymine-specific fluorescent stain.

• Sinónimos: Bisbenzimide H 33258 Fluorochrome, Trihydrochloride, 2-[2-(4-Hydroxyphenyl)-6-benzimidazoyl]-6-(1-methyl-4-piperazyl)-benzimidazole, 3HCl, HOE 33258
• Fórmula empírica (notación de Hill): C₂₅H₂₄N₆O · 3HCl
• Número de CAS: 23491-45-4
• Peso molecular: 533.88
• Número MDL: MFCD00012679
• Código UNSPSC: 12352111
• NACRES: NA.21

Propiedades

• Nivel de calidad: 100
• Análisis: ≥98% (HPLC)
• formulario: solid
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze; protect from light
• color: yellow-green
• fluorescencia: λ_ex 346 nm; λ_em 460 nm
• Condiciones de envío: ambient
• temp. de almacenamiento: 2-8°C
• InChI: 1S/C25H24N6O.3ClH/c1-30-10-12-31(13-11-30)18-5-9-21-23(15-18)29-25(27-21)17-4-8-20-22(14-17)28-24(26-20)16-2-6-19(32)7-3-16;;;/h2-9,14-15,26,28H,10-13H2,1H3,(H,27,29);3*1H
• Clave InChI: OKQAASMPIOSOEX-UHFFFAOYSA-N

Descripción

Descripción general

Membrane-permeable, adenine-thymine-specific fluorescent stain. Useful for staining DNA, chromosomes, and nuclei. Increases the rate of channel-mediated Ca²⁺ efflux from junctional cytoplasmic reticulum vesicles. Can also be used to detect mycoplasma contamination in tissue culture. H 33258 and the related compound H 33342 (Cat. No. 382065) have been used in conjunction with RNA aptamers in the 5′ untranslated region (UTR) to control expression of transfected gene constructs.

Membrane-permeant, adenine-thymidine specific fluorescent stain (excitation maximum: 346 nm; emission maximum: 460 nm). Useful for staining DNA, chromosomes, and nuclei. Increases the rate of channel-mediated Ca²⁺ efflux from junctional cytoplasmic reticulum vesicles. Can also be used to detect mycoplasma contamination in tissue culture. H 33258 and the related compound H33342 (Cat. No. 382065) have been used in conjunction with RNA aptamers in the 5′ untranslated region (UTR) to control expression of transfected gene constructs.

Acciones bioquímicas o fisiológicas

Cell permeable: yes

Primary Target
Adenine-thymine-specific fluorescent stain

Product does not compete with ATP.

Reversible: no

Advertencia

Toxicity: Carcinogenic / Teratogenic (D)

Nota de preparación

Precipitates in phosphate buffered solutions

Reconstitución

Following reconstitution, store in the refrigerator (4°C). Aqueous stock solutions are stable for up to 2 weeks at 4°C.

Otras notas

Werstuck, G., and Green, M.R. 1998. Science 282, 296.
Battaglio, M., et al. 1994. Biotech. Histochem. 69, 152.
Elstein, K.H., and Zucker, R.M. 1994. Exp. Cell Res. 211, 322.
Tarshis, M., et al. 1994. J. Immunol. Methods168, 245.
Beeler, T.J., and Gable, K. 1993. J. Membr. Biol. 135, 109.
Oberhammer, F., et al. 1993. EMBO J. 12, 3679.
Papadimitriou, E., and Lelkes, P.I. 1993. J. Immunol. Methods162, 41.
Jin, R., and Breslauer, K.J. 1988. Proc. Natl. Acad. Sci. USA85, 8939.
Cesarone, C.F., et al. 1979. Anal. Biochem.100, 188.
Latt, S.A., and Stetten, G. 1976. J. Histochem. Cytochem.24, 24.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable