220477-M

Chitosanase, Streptomyces sp. N174

Chitosanase, Streptomyces sp. N174, CAS 51570-20-8, is a member of a new class of enzymes with antifungal properties. Efficiently catalyzes the hydrolysis of acetylated chitosan (0 to 60%).

• Sinónimos: Chitosanase, Streptomyces sp. N174, Chitosan N-acetylglucosaminohydrolase
• Número de CAS: 51570-20-8
• Comisión internacional de enzimas: 3.2.1.132 (BRENDA, IUBMB)
• Número MDL: MFCD00211797
• Código UNSPSC: 12352202

Propiedades

• Nivel de calidad: 100
• Formulario: liquid
• actividad específica: ≥25 units/mg protein
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze; avoid repeated freeze/thaw cycles
• pI: 7.5 (temperature optimum 65°C for 10 minutes; recommended temperature 37°C)
• actividad extraña: chitinolytic ≤0.01%; proteases <0.25 Azocasein U/ml
• temp. de almacenamiento: −20°C

Descripción

Descripción general

Chitosanase, Streptomyces sp. N174, CAS 51570-20-8, is a member of a new class of enzymes with antifungal properties. Efficiently catalyzes the hydrolysis of acetylated chitosan (0 to 60%).

Native Chitosanase from Streptomyces species. A member of a new class of enzymes with antifungal properties that efficiently catalyzes the hydrolysis of acetylated chitosan (0 to 60%). Does not hydrolyze chitin or carboxylmethylcellulose. Acts by splitting the β1,4-glycosidic linkages of GlcNAc-GlcN (K_m = 29.3 µg/ml) and GlcNAc-GlcN. Its activity generally increases with decreasing acetylation of chitosan. Suitable for the efficient preparation of protoplasts of Mucor and other Zygomycetes, allowing the genetic manipulation of these fungi.

Native Chitosanase from Streptomyces species. Chromatographically purified. Chitosanase is a member of a new class of enzymes with antifungal properties that efficiently catalyzes the hydrolysis of acetylated chitosan (0 to 60%). Does not hydrolyze chitin or carboxylmethyl cellulose. Acts by splitting the b1,4-glycosidic linkages of GlcN-GlcN (K_m = 29.3 µg/ml) and GlcNAc-GlcN. Its activity generally increases with decreasing acetylation of chitosan. Suitable for the efficient preparation of protoplasts of Mucor and other Zygomycetes, allowing the genetic manipulation of these fungi. Has an optimal pH of 5.5, an optimal temperature of 65°C for 10 min, and a recommended temperature of 37°C.

Advertencia

Toxicity: Standard Handling (A)

Definición de unidad

One Azocasein unit is defined as the amount of protease that will liberate sufficient pigment to increase the optical density at 366 nm by 0.01 after incubation for 30 minutes with Azocasein

One unit is defined as the amount of enzyme that will release 1.0 µmol reducing sugars or glucosamine from chitosan per min at 37°C, pH 5.5.

Forma física

In 50 M sodium acetate, 50% glycerol, pH 5.0.

Reconstitución

Following initial thaw, aliquot and freeze (-20°C).

Otras notas

Boucher, I., et al. 1995. J. Biol. Chem. 270, 31077.
Cuero, R.G., and Osuji, G.O. 1995. Food Addit. Contam. 12, 479.
Fukamizo, T., et al. 1995. Biochem J. 311, 377.
Li, T., et al. 1995. Plant Physiol. Biochem. 33, 599.
Fukamizo, T., et al. 1994. Biochim. Biophys. Acta 1205, 183.
Osswald, W.F., et al. 1994. Plant Cell Physiol. 35, 811.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1