219364

Cathepsin B, Human Liver

Cathepsin B, Human Liver, CAS 9047-22-7, is a purified native cathepsin B from human liver, purified by affinity chromatography. Upregulated in many types of tumors.

• Sinónimos: Cathepsin B, Human Liver
• Número de CAS: 9047-22-7
• Comisión internacional de enzimas: 3.4.22.1 (BRENDA, IUBMB)
• Código UNSPSC: 12352202
• NACRES: NA.77

Propiedades

• origen biológico: human liver
• Nivel de calidad: 100
• Ensayo: >95% (SDS-PAGE)
• Formulario: liquid
• actividad específica: ≥50 units/mg protein
• purificado por: affinity chromatography
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze; avoid repeated freeze/thaw cycles
• técnicas: activity assay: suitable
• intervalo de pH: 3.5—6.0
• idoneidad: suitable for molecular biology
• aplicaciones: life science and biopharma
• Condiciones de envío: wet ice
• temp. de almacenamiento: −70°C
• Información sobre el gen: human ... CTSB(1508)

Descripción

Descripción general

Research area: Cell Signaling

Cathepsin B, Human Liver, is a native cathepsin B from human liver, purified by affinity chromatography. Cathepsin B is a lysosomal protease with endo/exopeptidase and a carboxydipeptidase activity. It is also known as cysteine cathepsin and belongs to the C1 family of papain-like enzymes. Cathepsin B like H, L, C, X, F, O and V is ubiquitously expressed in human tissues.

Optimal pH: 3.5-6.0.

Aplicación

Cathepsin B, Human Liver has been used to assess its linker cleavage or proteolytic activity using an LC/MS MRM-based quantitation method.

Acciones bioquímicas o fisiológicas

Cathepsin B facilitates non-specific, bulk proteolysis within the acidic conditions of the endosomal/lysosomal compartment. Here, they break down both intracellular and extracellular proteins. Upregulation and secretion of cathepsin B have been shown to occur in many types of tumors and to correlate positively with their invasive and metastatic capabilities. Cathepsin B is shown to facilitate tumor invasion by dissolving extracellular barriers.

Envase

Please refer to vial label for lot-specific concentration.

Advertencia

Toxicity: Standard Handling (A)

Definición de unidad

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Z-RR-βNA per min at 40°C, pH 6.0 in the presence of DTT.

Forma física

In 50 mM sodium acetate buffer, 1 mM EDTA, pH 5.0.

Nota de preparación

Prepared from tissue of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitución

Following initial thaw, aliquot and freeze (-70°C).

Otras notas

Kostoulas, G., et al. 1999. FEBS Lett. 455, 286.
Berquim, I.M., and Sloane, B.F. 1996. Adv. Exp. Med. Biol.389, 281.
Keppler, D., et al. 1994. Biochim. Biophys. Acta.1226, 117.
Musil, D., et al. 1991. EMBO J.10, 2321.
Sloane B.F. 1990. Semin. Cancer Biol.1, 137.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable