07-896-I

Anti-phospho-Rac1/cdc42 (Ser71) Antibody

from rabbit, purified by affinity chromatography

• Sinónimos: Ras-related C3 botulinum toxin substrate 1, Cell migration-inducing gene 5 protein, Ras-like protein TC25, p21-Rac1, phospho-Rac1/cdc42 (Ser71)
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: human
• reactividad de especies (predicha por homología): rat (based on 100% sequence homology), zebrafish (based on 100% sequence homology), orangutan (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology), Xenopus (based on 100% sequence homology), mouse (based on 100% sequence homology), chicken (based on 100% sequence homology)
• técnicas: immunoprecipitation (IP): suitable; western blot: suitable
• Nº de acceso NCBI: NP_008839
• Nº de acceso UniProt: P63000
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: phosphorylation (pSer71 )
• Información sobre el gen: human ... RAC1(5879)

Descripción

Descripción general

Target of rapamycin complex 2 subunit MAPKAP1 (UniProt Q9BPZ7; also known as MEKK2-interacting protein 1, Mitogen-activated protein kinase 2-associated protein 1, mSIN1, Ras inhibitor MGC2745, SAPK-interacting protein 1, Stress-activated protein kinase-interacting 1, Stress-activated map kinase-interacting protein 1,, TORC2 subunit MAPKAP1) is encoded by the MAPKAP1 (also known as JC310, MIP1 SIN1, SIN1b, SIN1g) gene (Gene ID 79109) in human. mSIN1 is an essential component of the Torc2 complex. mTORC2 is comprised of mTOR, a large Ser/Thr protein kinase, along with mSIN1, GbL (mLST8), Protor1, Protor2, and Rictor. The mTORC2 complex is activated primarily downstream of PI3 Kinase and is known to affect cell proliferation and survival primarily by phosphorylating Akt on Ser473. Additionally, the mTORC2 complex is known to affect cytoskeletal organization and cell migration by exerting its effects through Rac, Rho, and PKC. mSIN1 is also known to directly interact with Ras, SPAKs, and JNK.

Inmunógeno

Epitope: Near N-Terminus

KLH-conjugated linear peptide corresponding to human phospho-Rac1/cdc42 (Ser71) near the N-Terminus.

Aplicación

Detect Rac1/CDC42 using this rabbit Polyclonal Anti-phospho-Rac1/cdc42 (Ser71) Antibody, Cat. No. 07-896-I, validated for use in Western Blotting, Immunoprecipitation.

Immunoprecipitation Analysis: 2.5µg from a representative lot immunoprecipitated phospho-Rac1/cdc42 (Ser71) in EGF treated A431 cell lysate.

Research Category
Signaling

Research Sub Category
Signaling Neuroscience

Calidad

Evaluated by Western Blotting in EGF treated A431 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected phospho-Rac1/cdc42 (Ser71) in 10 µg of EGF treated A431 cell lysate.

Descripción de destino

~25 kDa observed

Forma física

Affinity purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable