07-523
Anti-trimethyl-Histone H3 (Lys9) Antibody
serum, Upstate®
Sinónimos:
H3K9me3, Histone H3 (tri methyl K9), H3 histone, family 3A
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
origen biológico
rabbit
Nivel de calidad
forma del anticuerpo
serum
tipo de anticuerpo
primary antibodies
clon
polyclonal
reactividad de especies
human
fabricante / nombre comercial
Upstate®
técnicas
dot blot: suitable
inhibition assay: suitable (peptide)
western blot: suitable
isotipo
IgG
Nº de acceso NCBI
Nº de acceso UniProt
Condiciones de envío
dry ice
modificación del objetivo postraduccional
trimethylation (Lys9)
Información sobre el gen
human ... H3F3B(3021)
Descripción general
Histone H3 is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Especificidad
A broad species cross-reactivity is expected.
Recognizes Histone H3 containing trimethyl-lysine 9 and, to a lesser extent, dimethyl-lysine 9. MW ~17 kDa.
Inmunógeno
BSA-conjugated, synthetic peptide containing the sequence …AR[me3K]S… in which me3K corresponds to trimethyl-lysine 9 of human Histone H3.
Epitope: Trimethyl Lys9
Aplicación
Anti-trimethyl-Histone H3 (Lys9) Antibody is a Rabbit Polyclonal Antibody for detection of trimethyl-Histone H3 (Lys9) also known as H3K9me3, Histone H3 (tri methyl K9) & has been validated in DB, PIA & WB.
Peptide Inhibition Analysis:
10 μM of a Histone H3 peptide containing trimethyl-lysine 9 abolished detection of Histone H3 by anti-trimethyl Histone H3 (Lys9) in western blot analysis of HeLa acid extracts (Figure B, lane 4). Slight signal reduction was observed with 10 μM of a Histone H3 peptide containing dimethyl-lysine 9.
Peptide Dot Blot Analysis:
A 1:2000 dilution of a previous lot was incubated with serial dilutions of Histone H3 peptides containing various modifications spotted onto PVDF. Slight cross-reactivity with Histone H3 peptide containing dimethyl-lysine 9 was detected.
Beadlyte Histone Peptide Assay:
1:2000-1:6000 dilutions of a previous lot were incubated with Histone H3 peptides containing various modifications conjugated to Luminex microspheres. Slight cross-reactivity with Histone H3 peptides containing dimethyl-lysine 9 and trimethyl-lysine 27 was detected.
10 μM of a Histone H3 peptide containing trimethyl-lysine 9 abolished detection of Histone H3 by anti-trimethyl Histone H3 (Lys9) in western blot analysis of HeLa acid extracts (Figure B, lane 4). Slight signal reduction was observed with 10 μM of a Histone H3 peptide containing dimethyl-lysine 9.
Peptide Dot Blot Analysis:
A 1:2000 dilution of a previous lot was incubated with serial dilutions of Histone H3 peptides containing various modifications spotted onto PVDF. Slight cross-reactivity with Histone H3 peptide containing dimethyl-lysine 9 was detected.
Beadlyte Histone Peptide Assay:
1:2000-1:6000 dilutions of a previous lot were incubated with Histone H3 peptides containing various modifications conjugated to Luminex microspheres. Slight cross-reactivity with Histone H3 peptides containing dimethyl-lysine 9 and trimethyl-lysine 27 was detected.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Calidad
Evaluated by western blot in acid-extracted proteins from HeLa cells.
Western Blot Analysis:
1:500-1:5000 dilution of this antibody detected methylated Histone H3 (Lys9) in acid-extracted proteins from HeLa cells. The antibody did not detect unmethylated recombinant Histone H3 (Catalog # 14-411).
Western Blot Analysis:
1:500-1:5000 dilution of this antibody detected methylated Histone H3 (Lys9) in acid-extracted proteins from HeLa cells. The antibody did not detect unmethylated recombinant Histone H3 (Catalog # 14-411).
Descripción de destino
~17 kDa
Forma física
Immunodepleted Serum
Rabbit polyclonal antiserum IgG in buffer containing 0.05% sodium azide before the addition of 30% glycerol.
Almacenamiento y estabilidad
Stable for 1 year at -20ºC from date of receipt.
Nota de análisis
Control
Acid extracted proteins from HeLa cells.
Acid extracted proteins from HeLa cells.
Otras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Información legal
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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