06-445

Anti-Kip1 (p27) Antibody

Upstate^®, from rabbit

• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• reactividad de especies: rabbit, human, rat
• envase: antibody small pack of 25 μg
• fabricante / nombre comercial: Upstate^®
• técnicas: immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotipo: IgG
• Nº de acceso NCBI: NM_004064
• Nº de acceso UniProt: P46527
• Condiciones de envío: dry ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... CDKN1B(1027)

Descripción

Descripción general

p27(Kip1) is a cyclin-dependent kinase inhibitor involved in G1 arrest. p27(Kip1) binds to and inhibits cyclinE-Cdk2 complex, cyclinA-CDK2 and cyclinD1-CDK4. p27(Kip1) is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27(Kip1); while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm.

Especificidad

Recognizes Kip1.

Inmunógeno

An 18 amino acid synthetic peptide ([C]GSVEQTPKKPGLRRRQT) linked to KLH was used to immunize rabbits

Aplicación

Anti-Kip1 (p27) Antibody is a high quality Rabbit Polyclonal Antibody for the detection of Kip1 (p27) & has been validated in IHC, IP & WB.

Calidad

routinely evaluated by immunoblot on HeLa nuclear extract or in rabbit brain nuclear extract

Descripción de destino

27 kDa

Ligadura / enlace

Replaces: 04-240

Forma física

Format: Purified

Nota de análisis

Control
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany