06-172-I

Anti-Na+/K+ ATPase alpha-3

from rabbit

• Sinónimos: ATP1A3, Na+/K+ ATPase alpha-3, Sodium/potassium-transporting ATPase subunit alpha-3, Na(+)/K(+) ATPase alpha-3 subunit, Na(+)/K(+) ATPase alpha(III) subunit, Sodium pump subunit alpha-3
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• reactividad de especies: human
• reactividad de especies (predicha por homología): mouse (based on 100% sequence homology), rat (based on 100% sequence homology)
• envase: antibody small pack of 25 μL
• técnicas: immunohistochemistry: suitable (paraffin); western blot: suitable
• isotipo: IgG
• Nº de acceso NCBI: NP_036638
• Nº de acceso UniProt: P06687
• Condiciones de envío: ambient
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: rat ... Atp1A1(24211)

Descripción

Descripción general

Sodium/potassium-transporting ATPase subunit alpha-3 (UniProt: P06687; also known as EC: 3.6.3.9, Na(+)/K(+) ATPase alpha-3 subunit, Na(+)/K(+) ATPase alpha(III) subunit, Sodium pump subunit alpha-3) is encoded by the Atp1a3 gene (Gene ID: 24213) in rat. Sodium/potassium-transporting ATPase subunit alpha-3 is a multi-pass membrane protein that is exclusive to the brain tissue and is highly expressed in cell bodies and projections of hippocampus. It is responsible for establishing and maintaining the electrochemical gradients of sodium and potassium ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. Sodium/potassium-transporting ATPase subunit alpha-3 is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic component catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. Reduced levels of neuronal sodium/ potassium ATPase activity in ATP1A3 heterozygous mice increases their susceptibility to depression-like endophenotypes by chronic variable stress.

Especificidad

This rabbit polyclonal antibody detects Sodium/potassium-transporting ATPase subunit alpha-3 in human, mouse, and rat brain. It targets an epitope with in 25 amino acids from the N-terminal region.

Inmunógeno

Epitope: N-terminus

KLH-conjugated linear peptide corresponding to 25 amino acids from the N-terminal region of rat Sodium/potassium-transporting ATPase subunit alpha-3.

Aplicación

Anti-Na+/K+ ATPase alpha-3, Cat. No. 06-172-I, is a highly specific rabbit polyclonal antibody that targets Sodium/potassium-transporting ATPase subunit alpha-3 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:250-1,000 dilution of this antibody detected Na+/K+ ATPase alpha-3 in human cerebral cortex and human cardiac muscle tissue.

Research Category
Neuroscience

Calidad

Evaluated by Western Blotting in adult human brain tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Na+/K+ ATPase alpha-3 in 10 µg of adult human brain tissue lysate.

Descripción de destino

~112 kDa observed; 111.75 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Forma física

Affinity Purified

Format: Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1