05-784R

Anti-IRS1 Antibody, clone 58-10C-31, rabbit monoclonal

clone 58-10C-31, from rabbit

• Sinónimos: insulin receptor substrate 1
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: 58-10C-31, monoclonal
• reactividad de especies: human, rat, mouse
• técnicas: immunoprecipitation (IP): suitable; western blot: suitable
• isotipo: IgG
• Nº de acceso NCBI: NP_0371
• Nº de acceso UniProt: P35570
• Condiciones de envío: dry ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... IRS1(3667)

Descripción

Descripción general

IRS1 (Insulin Receptor Substrate 1) transmits insulin signals via metabolic and mitogenic pathways. It is heavily phosphorylated on both serine and tyrosine residues. These phosphorylated tyrosines enable IRS to act as a docking protein that binds SH2 domains of such proteins as PI3 Kinase (phosphatidylinositol 3-kinase) and GRB2, resulting in activation. Over expression and phosphorylation of serine is associated with insulin resistance and breast cancer.

Especificidad

Antibody recognizes the C-terminus of IRS1.

Inmunógeno

Epitope: C-terminus

KLH- conjugated - linear peptide corresponding to the C-terminus of rat IRS1.

Aplicación

Detect IRS1 using this Anti-IRS1 Antibody, clone 58-10C-31 validated for use in WB & IP.

Research Category
Metabolism

Research Sub Category
Insulin/Energy Signaling

Western Blot (SNAP ID) Analysis: 0.1 µg/mL from a previous lot detected IRS-1 on 10 µg of 3T3/A31, 3T3/L1, L6 and MCF7 cell lysates.

Immunoprecipitation Analysis: 1 µg from a previous lot immunoprecipitated IRS-1 from 100 µg of MCF7 cell lysate. Arrow indicates IRS-1 (~160 kDa). There is cross reactivity of the detection antibody with the heavy chaing of Rabbit IgG as shown at ~50 kDa.

Calidad

Evaluated by Western Blot in 3T3/A31, 3T3/L1, L6 or MCF7 cell lysates.

Western Blot Analysis: 0.1 µg/mL of this antibody detected IRS-1 on 10 µg of 3T3/A31, 3T3/L1, L6, or MCF7 cell lysates.

Descripción de destino

~ 160 kDa

Forma física

Format: Purified

Protein A purified

Purified Rabbit Monoclonal IgG Supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide and 40% glycerol.

Almacenamiento y estabilidad

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Nota de análisis

Control
3T3/A31, 3T3/L1, L6, or MCF7 cell lysates

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable