05-157
Anti-MAPK1/ERK2 Antibody
Upstate, mouse monoclonal, 1B3B9
Sinónimos:
Extracellular signal-regulated kinase 2, MAP kinase 2, MAPK 2, Mitogen-activated protein kinase 2, extracellular signal-regulated kinase-2, mitogen-activated protein kinase 1, protein tyrosine kinase ERK2.
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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Nombre del producto
Anti-MAP Kinase 2/Erk2 Antibody, clone 1B3B9, clone 1B3B9, Upstate®, from mouse
origen biológico
mouse
Nivel de calidad
forma del anticuerpo
purified immunoglobulin
tipo de anticuerpo
primary antibodies
clon
1B3B9, monoclonal
reactividad de especies
avian, mouse, human, rat
fabricante / nombre comercial
Upstate®
técnicas
immunoprecipitation (IP): suitable
western blot: suitable
isotipo
IgG2a
Nº de acceso NCBI
Nº de acceso UniProt
Condiciones de envío
dry ice
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... MAPKAPK2(9261)
mouse ... Mapkapk2(17164)
rat ... Mapkapk2(289014)
Descripción general
ERK2 is activated by MEK2, which phosphorylates neighboring threonine 183 and tyrosine 185 residues. ERK2 is a serine/threonine kinase that phosphorylates MAP2 and myelin basic protein. This kinase is an important proximal component of the MAP kinase pathway involved in transeitting the signals from growth factors, neurotransmitters and hormones at the cell surface to the transcriptional events in the nucleus.
Especificidad
Other species cross reactivity is unknown.
This antibody recognizes MAP kinase encoded by the mapk gene, Mr 42 kDa.
Inmunógeno
HPLC purified murine recombinant MAP Kinase. Clone 1B3B9
Aplicación
Anti-MAP Kinase 2/Erk2 Antibody, clone 1B3B9 is an antibody against MAP Kinase 2/Erk2 for use in IP & WB.
Immunoprecipitation:
4 μg of a previous lot immunoprecipitated MAP Kinase 2/Erk2 from a RIPA lysate from mouse 3T3 cells. Boil the cell lysate and let cool before adding the antibody, as this antibody only binds to denatured MAP Kinase.
4 μg of a previous lot immunoprecipitated MAP Kinase 2/Erk2 from a RIPA lysate from mouse 3T3 cells. Boil the cell lysate and let cool before adding the antibody, as this antibody only binds to denatured MAP Kinase.
Research Category
Signaling
Signaling
Research Sub Category
MAP Kinases
MAP Kinases
Calidad
Routinely evaluated in RIPA lysates from human A431 carcinoma cells, mouse 3T3 fibroblasts, or rat L6 cells.
Western Blot Analysis:
0.5-2 µg/mL of this lot detected MAP Kinase 2/Erk2 in RIPA lysates from human A431 carcinoma cells. A previous lot detected MAP Kinase in mouse 3T3 fibroblasts and rat L6 cells.
Western Blot Analysis:
0.5-2 µg/mL of this lot detected MAP Kinase 2/Erk2 in RIPA lysates from human A431 carcinoma cells. A previous lot detected MAP Kinase in mouse 3T3 fibroblasts and rat L6 cells.
Descripción de destino
42 kDa
Ligadura / enlace
Replaces: 04-349
Forma física
Format: Purified
Protein G Chromatography
Purified mouse monoclonal IgG2a in buffer containing PBS with 0.05% sodium azide. Store frozen at -20°C, avoid freeze thaw cycles.
Almacenamiento y estabilidad
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Nota de análisis
Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Otras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Información legal
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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