05-1516

Anti-hnRNP U Antibody, clone 3G6

clone 3G6, from mouse

• Sinónimos: Scaffold attachment factor A, heterogeneous nuclear ribonucleoprotein U, heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A), hnRNP U, hnRNP U protein, p120 nuclear protein
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: 3G6, monoclonal
• reactividad de especies: human
• técnicas: ELISA: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotipo: IgG2bκ
• Nº de acceso NCBI: NP_004492.2
• Nº de acceso UniProt: Q00839
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... HNRNPU(3192)

Descripción

Descripción general

Heterogeneous nuclear ribonucleoproteins (hnRNPs) are thought to be involved in pre-mRNA processing. However, its role in the regulation of gene expression is as yet poorly understood. Proteins of the heterogeneous nuclear ribonucleoparticles (hnRNP) family form a structurally diverse group of RNA binding proteins implicated in various functions. Recently, hnRNP proteins have been shown to hinder communication between factors bound to different splice sites. Conversely, several reports have described a positive role for some hnRNP proteins in pre-mRNA splicing. hnRNP-U, also termed scaffold attachment factor A (SAF-A), binds to pre-mRNA and nuclear matrix/scaffold attachment region DNA elements.

Especificidad

This antibody recognizes hnRNP U.

Inmunógeno

Epitope: Unknown

Recombinant protein corresponding to human hnRNP U.

Aplicación

Immunoprecipitation
Reported by external laboratory using a represenative lot of this antibody
Immunocytochemistry
Reported by external laboratory using a represenative lot of this antibody
ELISA
Reported by external laboratory using a represenative lot of this antibody

Research Category
Epigenetics & Nuclear Function

Research Sub Category
RNA Metabolism & Binding Proteins

Apoptosis - Additional

Use Anti-hnRNP U Antibody, clone 3G6 (mouse monoclonal antibody) validated in WB, IP, ICC, ELISA to detect hnRNP U also known as heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A).

Calidad

Evaluated by Western Blot in K562 cell lysate.

Western Blot Analysis: 0.01 µg/ml of this antibody detected hnRNP U on 10 µg of K562 cell lysate.

Descripción de destino

Calculated molecular weight is 90 kDa However protein is usually observed at ~120 kDa (Dreyfuss G, Kim VN, Kataoka N. Nat Rev Mol Cell Biol. 2002 Mar;3(3):195-205.)

Forma física

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Nota de análisis

Control
K562 cell lysate

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable