05-1354
Anti-Histone H3 Antibody (Dimethyl K9, Phospho S10), clone 6HH3-2C5
ascites fluid, clone 6HH3-2C5, from mouse
Sinónimos:
H3K9me2S10P, Histone H3 (di methyl K9, phospho S10), H3 histone, family 3B (H3.3B)
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
origen biológico
mouse
Nivel de calidad
forma del anticuerpo
ascites fluid
tipo de anticuerpo
primary antibodies
clon
6HH3-2C5, monoclonal
reactividad de especies
human
reactividad de especies (predicha por homología)
Drosophila (based on 100% sequence homology)
técnicas
immunocytochemistry: suitable
western blot: suitable
isotipo
IgG1κ
Nº de acceso GenBank
Nº de acceso UniProt
Condiciones de envío
wet ice
modificación del objetivo postraduccional
dimethylation (Lys9), phosphorylation (pSer10)
Información sobre el gen
human ... H3F3B(3021)
Categorías relacionadas
Descripción general
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Histone proteins are highly post-translationally modified, and Histone H3 is the most extensively modified of the five histones. The term "Histone H3" alone is purposely ambiguous in that it does not distinguish between sequence variants or modification state. Histone H3 is an important protein in the emerging field of epigenetics, where its sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes.
Especificidad
Antibody recognizes dimethyl (Lys9) phospho(Ser10) on Histone H3.
Inmunógeno
Epitope: Dimethyl (Lys9) & Phospho (Ser10)
Synthetic linear peptide corresponding to dimethyl (Lys9) and Phospho (Ser10) of Histone H3.
Aplicación
Immunocytochemistry:
A 1:500 dilution of a representative lot of this antibody detected Dimethyl (Lys9)-Phospho (Ser10) Histone H3 in A431 and HeLa cells.
A 1:500 dilution of a representative lot of this antibody detected Dimethyl (Lys9)-Phospho (Ser10) Histone H3 in A431 and HeLa cells.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Use Anti-Histone H3 Antibody (Dimethyl K9, Phospho S10), clone 6HH3-2C5 (mouse monoclonal antibody) validated in WB, ICC to detect Histone H3 (Dimethyl K9 also known as H3K9me2S10P, Histone H3 (di methyl K9 phospho S10).
Calidad
Evaluated by western blot in untreated and Etoposide-treated HeLa acid extracts.
Western Blot Analysis:
A 1:1,000 dilution of this antibody detected Dimethyl (Lys9)-Phospho (Ser10) Histone H3 in 10 µg of untreated and Etoposide-treated HeLa acid extracts.
Western Blot Analysis:
A 1:1,000 dilution of this antibody detected Dimethyl (Lys9)-Phospho (Ser10) Histone H3 in 10 µg of untreated and Etoposide-treated HeLa acid extracts.
Descripción de destino
~17 kDa
Forma física
Mouse monoclonal IgG1κ ascites with 0.05% sodium azide.
Unpurified
Almacenamiento y estabilidad
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Nota de análisis
Control
Untreated and Etoposide-treated HeLa acid extracts.
Untreated and Etoposide-treated HeLa acid extracts.
Información legal
GenBank is a registered trademark of United States Department of Health and Human Services
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
nwg
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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